Abstract: Pedestrian detection is of paramount interest for many applications, e.g. Advanced Driver Assistance Systems, Intelligent Video Surveillance and Multimedia systems. Most promising pedestrian detectors rely on appearance-based classifiers trained with annotated data. However, the required annotation step represents an intensive and subjective task for humans, what makes worth to minimize their intervention in this process by using computational tools like realistic virtual worlds. The reason to use these kind of tools relies in the fact that they allow the automatic generation of precise and rich annotations of visual information. Nevertheless, the use of this kind of data comes with the following question: can a pedestrian appearance model learnt with virtual-world data work successfully for pedestrian detection in real-world scenarios?. To answer this question, we conduct different experiments that suggest a positive answer. However, the pedestrian classifiers trained with virtual-world data can suffer the so called dataset shift problem as real-world based classifiers does. Accordingly, we have designed different domain adaptation techniques to face this problem, all of them integrated in a same framework (V-AYLA). We have explored different methods to train a domain adapted pedestrian classifiers by collecting a few pedestrian samples from the target domain (real world) and combining them with many samples of the source domain (virtual world). The extensive experiments we present show that pedestrian detectors developed within the V-AYLA framework do achieve domain adaptation. Ideally, we would like to adapt our system without any human intervention. Therefore, as a first proof of concept we also propose an unsupervised domain adaptation technique that avoids human intervention during the adaptation process. To the best of our knowledge, this Thesis work is the first demonstrating adaptation of virtual and real worlds for developing an object detector. Last but not least, we also assessed a different strategy to avoid the dataset shift that consists in collecting real-world samples and retrain with them in such a way that no bounding boxes of real-world pedestrians have to be provided. We show that the generated classifier is competitive with respect to the counterpart trained with samples collected by manually annotating pedestrian bounding boxes. The results presented on this Thesis not only end with a proposal for adapting a virtual-world pedestrian detector to the real world, but also it goes further by pointing out a new methodology that would allow the system to adapt to different situations, which we hope will provide the foundations for future research in this unexplored area.

Abstract: This paper presents an approach to mitigate the contribution of blood vessels to the energy image used at different tasks of automatic colonoscopy image analysis. This goal is achieved by introducing a characterization of endoluminal scene objects which allows us to differentiate between the trace of 2-dimensional visual objects,such as vessels, and shades from 3-dimensional visual objects, such as folds. The proposed characterization is based on the influence that the object shape has in the resulting visual feature, and it leads to the development of a blood vessel attenuation algorithm. A database consisting of manually labelled masks was built in order to test the performance of our method, which shows an encouraging success in blood vessel mitigation while keeping other structures intact. Moreover, by extending our method to the only available polyp localization
algorithm tested on a public database, blood vessel mitigation proved to have a positive influence on the overall performance.

Abstract: The increasing ubiquitousness of digital information in our daily lives has positioned video as a favored information vehicle, and given rise to an astonishing generation of social media and surveillance footage. This raises a series of technological demands for automatic video understanding and management, which together with the compromising attentional limitations of human operators, have motivated the research community to guide its steps towards a better attainment of such capabilities. As a result, current trends on cognitive vision promise to recognize complex events and self-adapt to different environments, while managing and integrating several types of knowledge. Future directions suggest to reinforce the multi-modal fusion of information sources and the communication with end-users.

Abstract: Aggregates of misfolded proteins are a hallmark of many age-related diseases. Recently, they have been linked to aging of Escherichia coli (E. coli) where protein aggregates accumulate at the old pole region of the aging bacterium. Because of the potential of E. coli as a model organism, elucidating aging and protein aggregation in this bacterium may pave the way to significant advances in our global understanding of aging. A first obstacle along this path is to decipher the mechanisms by which protein aggregates are targeted to specific intercellular locations. Here, using an integrated approach based on individual-based modeling, time-lapse fluorescence microscopy and automated image analysis, we show that the movement of aging-related protein aggregates in E. coli is purely diffusive (Brownian). Using single-particle tracking of protein aggregates in live E. coli cells, we estimated the average size and diffusion constant of the aggregates. Our results provide evidence that the aggregates passively diffuse within the cell, with diffusion constants that depend on their size in agreement with the Stokes-Einstein law. However, the aggregate displacements along the cell long axis are confined to a region that roughly corresponds to the nucleoid-free space in the cell pole, thus confirming the importance of increased macromolecular crowding in the nucleoids. We thus used 3D individual-based modeling to show that these three ingredients (diffusion, aggregation and diffusion hindrance in the nucleoids) are sufficient and necessary to reproduce the available experimental data on aggregate localization in the cells. Taken together, our results strongly support the hypothesis that the localization of aging-related protein aggregates in the poles of E. coli results from the coupling of passive diffusion-aggregation with spatially non-homogeneous macromolecular crowding. They further support the importance of “soft” intracellular structuring (based on macromolecular crowding) in diffusion-based protein localization in E. coli.

Abstract: Vision-based road detection is important for different applications in transportation, such as autonomous driving, vehicle collision warning, and pedestrian crossing detection. Common approaches to road detection are based on low-level road appearance (e.g., color or texture) and neglect of the scene geometry and context. Hence, using only low-level features makes these algorithms highly depend on structured roads, road homogeneity, and lighting conditions. Therefore, the aim of this paper is to classify road geometries for road detection through the analysis of scene composition and temporal coherence. Road geometry classification is proposed by building corresponding models from training images containing prototypical road geometries. We propose adaptive shape models where spatial pyramids are steered by the inherent spatial structure of road images. To reduce the influence of lighting variations, invariant features are used. Large-scale experiments show that the proposed road geometry classifier yields a high recognition rate of 73.57% ± 13.1, clearly outperforming other state-of-the-art methods. Including road shape information improves road detection results over existing appearance-based methods. Finally, it is shown that invariant features and temporal information provide robustness against disturbing imaging conditions.

Abstract: The operational model of agonism assumes that the maximum effect a particular receptor system can achieve (the Em parameter) is fixed. Em estimates are above but close to the asymptotic maximum effects of endogenous agonists. The concept of Em is contradicted by superagonists and those positive allosteric modulators that significantly increase the maximum effect of endogenous agonists. An extension of the operational model is proposed that assumes that the Em parameter does not necessarily have a single value for a receptor system but has multiple values associated to multiple active receptor conformations. The model provides a mechanistic link between active receptor conformation and agonist efficacy, which can be useful for the analysis of agonist response under different receptor scenarios.

Abstract: In this paper, the registration problem is formulated as a point to model distance minimization. Unlike most of the existing works, which are based on minimizing a point-wise correspondence term, this formulation avoids the correspondence search that is time-consuming. In the first stage, the target set is described through an implicit function by employing a linear least squares fitting. This function can be either an implicit polynomial or an implicit B-spline from a coarse to fine representation. In the second stage, we show how the obtained implicit representation is used as an interface to convert point-to-point registration into point-to-implicit problem. Furthermore, we show that this registration distance is smooth and can be minimized through the Levengberg-Marquardt algorithm. All the formulations presented for both stages are compact and easy to implement. In addition, we show that our registration method can be handled using any implicit representation though some are coarse and others provide finer representations; hence, a tradeoff between speed and accuracy can be set by employing the right implicit function. Experimental results and comparisons in 2D and 3D show the robustness and the speed of convergence of the proposed approach.