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Author Patricia Marquez; Debora Gil; R.Mester; Aura Hernandez-Sabate edit   pdf
openurl 
  Title Local Analysis of Confidence Measures for Optical Flow Quality Evaluation Type Conference Article
  Year 2014 Publication (up) 9th International Conference on Computer Vision Theory and Applications Abbreviated Journal  
  Volume 3 Issue Pages 450-457  
  Keywords Optical Flow; Confidence Measure; Performance Evaluation.  
  Abstract Optical Flow (OF) techniques facing the complexity of real sequences have been developed in the last years. Even using the most appropriate technique for our specific problem, at some points the output flow might fail to achieve the minimum error required for the system. Confidence measures computed from either input data or OF output should discard those points where OF is not accurate enough for its further use. It follows that evaluating the capabilities of a confidence measure for bounding OF error is as important as the definition
itself. In this paper we analyze different confidence measures and point out their advantages and limitations for their use in real world settings. We also explore the agreement with current tools for their evaluation of confidence measures performance.
 
  Address Lisboa; January 2014  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN ISBN Medium  
  Area Expedition Conference VISAPP  
  Notes IAM; ADAS; 600.044; 600.060; 600.057; 601.145; 600.076; 600.075 Approved no  
  Call Number Admin @ si @ MGM2014 Serial 2432  
Permanent link to this record
 

 
Author Patricia Marquez; Debora Gil ; Aura Hernandez-Sabate edit   pdf
doi  isbn
openurl 
  Title Error Analysis for Lucas-Kanade Based Schemes Type Conference Article
  Year 2012 Publication (up) 9th International Conference on Image Analysis and Recognition Abbreviated Journal  
  Volume 7324 Issue I Pages 184-191  
  Keywords Optical flow, Confidence measure, Lucas-Kanade, Cardiac Magnetic Resonance  
  Abstract Optical flow is a valuable tool for motion analysis in medical imaging sequences. A reliable application requires determining the accuracy of the computed optical flow. This is a main challenge given the absence of ground truth in medical sequences. This paper presents an error analysis of Lucas-Kanade schemes in terms of intrinsic design errors and numerical stability of the algorithm. Our analysis provides a confidence measure that is naturally correlated to the accuracy of the flow field. Our experiments show the higher predictive value of our confidence measure compared to existing measures.  
  Address Aveiro, Portugal  
  Corporate Author Thesis  
  Publisher Springer-Verlag Berlin Heidelberg Place of Publication Editor  
  Language english Summary Language Original Title  
  Series Editor Campilho, Aurélio and Kamel, Mohamed Series Title Lecture Notes in Computer Science Abbreviated Series Title LNCS  
  Series Volume Series Issue Edition  
  ISSN 0302-9743 ISBN 978-3-642-31294-6 Medium  
  Area Expedition Conference ICIAR  
  Notes IAM Approved no  
  Call Number IAM @ iam @ MGH2012a Serial 1899  
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Author Sergio Vera; Miguel Angel Gonzalez Ballester; Debora Gil edit   pdf
url  doi
isbn  openurl
  Title Optimal Medial Surface Generation for Anatomical Volume Representations Type Book Chapter
  Year 2012 Publication (up) Abdominal Imaging. Computational and Clinical Applications Abbreviated Journal LNCS  
  Volume 7601 Issue Pages 265-273  
  Keywords Medial surface representation; volume reconstruction  
  Abstract Medial representations are a widely used technique in abdominal organ shape representation and parametrization. Those methods require good medial manifolds as a starting point. Any medial
surface used to parametrize a volume should be simple enough to allow an easy manipulation and complete enough to allow an accurate reconstruction of the volume. Obtaining good quality medial
surfaces is still a problem with current iterative thinning methods. This forces the usage of generic, pre-calculated medial templates that are adapted to the final shape at the cost of a drop in volume reconstruction.
This paper describes an operator for generation of medial structures that generates clean and complete manifolds well suited for their further use in medial representations of abdominal organ volumes. While being simpler than thinning surfaces, experiments show its high performance in volume reconstruction and preservation of medial surface main branching topology.
 
  Address Nice, France  
  Corporate Author Thesis  
  Publisher Springer Berlin Heidelberg Place of Publication Editor Yoshida, Hiroyuki and Hawkes, David and Vannier, MichaelW.  
  Language Summary Language Original Title  
  Series Editor Series Title Lecture Notes in Computer Science Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0302-9743 ISBN 978-3-642-33611-9 Medium  
  Area Expedition Conference STACOM  
  Notes IAM Approved no  
  Call Number IAM @ iam @ VGG2012b Serial 1988  
Permanent link to this record
 

 
Author Enric Marti; Ferran Poveda; Antoni Gurgui; Debora Gil edit   pdf
url  isbn
openurl 
  Title Aprendizaje Basado en Proyectos en Ingeniería Informática. Resultados y reflexiones de seis años de experiencia Type Miscellaneous
  Year 2011 Publication (up) Actas del Simposio-Taller JENUI 2011 Abbreviated Journal  
  Volume Issue Pages 1-8  
  Keywords  
  Abstract In this workshop a 6 years experience in Project Based Learning (PBL) in Computer Graphics, Computer Engineering course at the Autonomous University of Barcelona (UAB) is presented. We use a Moodle environment suited to manage the documentation generated in PBL. The course is organized by means of two alternative routes: a classic itinerary of lectures and test-based evaluation and another with PBL. In the PBL itinerary we explain the organization in teamgroups, homework tutoring and monitoring and evaluation guidelines for students. We provide some of the work done by students, and the results of assessment surveys carried out to students during these years. We report the evolution of our PBL itinerary in terms of, both, organization and student surveys.
The workshop aims at discussing about on the advantages and disadvantages of using these active methodologies in technical degrees such as computer engineering, in order to debate about the most suitable way of organizing PBL and assessing students learning rate.
 
  Address Sevilla, Spain  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language spanish Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN ISBN 978-84-694-5440-4 Medium  
  Area Expedition Conference JENUI  
  Notes IAM Approved no  
  Call Number IAM @ iam @ MPG2011 Serial 1584  
Permanent link to this record
 

 
Author Enric Marti; Debora Gil; Marc Vivet ; Carme Julia edit   pdf
openurl 
  Title Balance de cuatro años de experiencia en la implantación de la metodología de Aprendizaje Basado en Proyectos en la asignatura de Gráficos por Computador en ingeniería Informática Type Miscellaneous
  Year 2008 Publication (up) Actas V Jornadas Internacionales de Innovación Universitaria Abbreviated Journal  
  Volume Issue Pages  
  Keywords Aprendizaje cooperativo; aprendizaje basado en proyectos; experiencias docentes.  
  Abstract En este trabajo se presentan los resultados de la aplicación de la metodología del aprendizaje cooperativo a la docencia de dos asignaturas de programación en ingeniería informática. ‘Algoritmos y programación’ y ‘Lenguajes de programación’ son dos asignaturas complementarias que se organizan entorno a un proyecto común que engloba los contenidos de ambas asignaturas. En la docencia de una parte muy importante de estas asignaturas, la metodología del aprendizaje cooperativo se ha adaptado a sus características específicas. Como muestra de esta adaptación presentamos dos ejemplos de las actividades desarrolladas dentro de la docencia de estas asignaturas. Después de tres años de aplicación, el análisis a nivel cualitativo y cuantitativo de los resultados muestra que éstos son muy satisfactorios y que la aplicación del método cooperativo ha mejorado de forma considerable el rendimiento de los alumnos en ambas asignaturas.  
  Address  
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  Publisher Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN ISBN Medium  
  Area Expedition Conference  
  Notes IAM;ADAS Approved no  
  Call Number IAM @ iam @ MGV2008a Serial 1598  
Permanent link to this record
 

 
Author Maurizio Mencuccini; Jordi Martinez-Vilalta; Josep Piñol; Lasse Loepfe; Mireia Burnat ; Xavier Alvarez; Juan Camacho; Debora Gil edit   pdf
url  doi
openurl 
  Title A quantitative and statistically robust method for the determination of xylem conduit spatial distribution Type Journal Article
  Year 2010 Publication (up) American Journal of Botany Abbreviated Journal AJB  
  Volume 97 Issue 8 Pages 1247-1259  
  Keywords Geyer; hydraulic conductivity; point pattern analysis; Ripley; Spatstat; vessel clusters; xylem anatomy; xylem network  
  Abstract Premise of the study: Because of their limited length, xylem conduits need to connect to each other to maintain water transport from roots to leaves. Conduit spatial distribution in a cross section plays an important role in aiding this connectivity. While indices of conduit spatial distribution already exist, they are not well defined statistically. * Methods: We used point pattern analysis to derive new spatial indices. One hundred and five cross-sectional images from different species were transformed into binary images. The resulting point patterns, based on the locations of the conduit centers-of-area, were analyzed to determine whether they departed from randomness. Conduit distribution was then modeled using a spatially explicit stochastic model. * Key results: The presence of conduit randomness, uniformity, or aggregation depended on the spatial scale of the analysis. The large majority of the images showed patterns significantly different from randomness at least at one spatial scale. A strong phylogenetic signal was detected in the spatial variables. * Conclusions: Conduit spatial arrangement has been largely conserved during evolution, especially at small spatial scales. Species in which conduits were aggregated in clusters had a lower conduit density compared to those with uniform distribution. Statistically sound spatial indices must be employed as an aid in the characterization of distributional patterns across species and in models of xylem water transport. Point pattern analysis is a very useful tool in identifying spatial patterns.  
  Address  
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  Publisher Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN ISBN Medium  
  Area Expedition Conference  
  Notes IAM; Approved no  
  Call Number IAM @ iam @ MMG2010 Serial 1623  
Permanent link to this record
 

 
Author Sonia Baeza; R.Domingo; M.Salcedo; G.Moragas; J.Deportos; I.Garcia Olive; Carles Sanchez; Debora Gil; Antoni Rosell edit  url
openurl 
  Title Artificial Intelligence to Optimize Pulmonary Embolism Diagnosis During Covid-19 Pandemic by Perfusion SPECT/CT, a Pilot Study Type Journal Article
  Year 2021 Publication (up) American Journal of Respiratory and Critical Care Medicine Abbreviated Journal  
  Volume Issue Pages  
  Keywords  
  Abstract  
  Address  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN ISBN Medium  
  Area Expedition Conference  
  Notes IAM; 600.145 Approved no  
  Call Number Admin @ si @ BDS2021 Serial 3591  
Permanent link to this record
 

 
Author Guillermo Torres; Sonia Baeza; Carles Sanchez; Ignasi Guasch; Antoni Rosell; Debora Gil edit  doi
openurl 
  Title An Intelligent Radiomic Approach for Lung Cancer Screening Type Journal Article
  Year 2022 Publication (up) Applied Sciences Abbreviated Journal APPLSCI  
  Volume 12 Issue 3 Pages 1568  
  Keywords Lung cancer; Early diagnosis; Screening; Neural networks; Image embedding; Architecture optimization  
  Abstract The efficiency of lung cancer screening for reducing mortality is hindered by the high rate of false positives. Artificial intelligence applied to radiomics could help to early discard benign cases from the analysis of CT scans. The available amount of data and the fact that benign cases are a minority, constitutes a main challenge for the successful use of state of the art methods (like deep learning), which can be biased, over-fitted and lack of clinical reproducibility. We present an hybrid approach combining the potential of radiomic features to characterize nodules in CT scans and the generalization of the feed forward networks. In order to obtain maximal reproducibility with minimal training data, we propose an embedding of nodules based on the statistical significance of radiomic features for malignancy detection. This representation space of lesions is the input to a feed
forward network, which architecture and hyperparameters are optimized using own-defined metrics of the diagnostic power of the whole system. Results of the best model on an independent set of patients achieve 100% of sensitivity and 83% of specificity (AUC = 0.94) for malignancy detection.
 
  Address Jan 2022  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN ISBN Medium  
  Area Expedition Conference  
  Notes IAM; 600.139; 600.145 Approved no  
  Call Number Admin @ si @ TBS2022 Serial 3699  
Permanent link to this record
 

 
Author Aura Hernandez-Sabate; Jose Elias Yauri; Pau Folch; Miquel Angel Piera; Debora Gil edit  doi
openurl 
  Title Recognition of the Mental Workloads of Pilots in the Cockpit Using EEG Signals Type Journal Article
  Year 2022 Publication (up) Applied Sciences Abbreviated Journal APPLSCI  
  Volume 12 Issue 5 Pages 2298  
  Keywords Cognitive states; Mental workload; EEG analysis; Neural networks; Multimodal data fusion  
  Abstract The commercial flightdeck is a naturally multi-tasking work environment, one in which interruptions are frequent come in various forms, contributing in many cases to aviation incident reports. Automatic characterization of pilots’ workloads is essential to preventing these kind of incidents. In addition, minimizing the physiological sensor network as much as possible remains both a challenge and a requirement. Electroencephalogram (EEG) signals have shown high correlations with specific cognitive and mental states, such as workload. However, there is not enough evidence in the literature to validate how well models generalize in cases of new subjects performing tasks with workloads similar to the ones included during the model’s training. In this paper, we propose a convolutional neural network to classify EEG features across different mental workloads in a continuous performance task test that partly measures working memory and working memory capacity. Our model is valid at the general population level and it is able to transfer task learning to pilot mental workload recognition in a simulated operational environment.  
  Address February 2022  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN ISBN Medium  
  Area Expedition Conference  
  Notes IAM; ADAS; 600.139; 600.145; 600.118 Approved no  
  Call Number Admin @ si @ HYF2022 Serial 3720  
Permanent link to this record
 

 
Author Jose Elias Yauri; Aura Hernandez-Sabate; Pau Folch; Debora Gil edit  doi
openurl 
  Title Mental Workload Detection Based on EEG Analysis Type Conference Article
  Year 2021 Publication (up) Artificial Intelligent Research and Development. Proceedings 23rd International Conference of the Catalan Association for Artificial Intelligence. Abbreviated Journal  
  Volume 339 Issue Pages 268-277  
  Keywords Cognitive states; Mental workload; EEG analysis; Neural Networks.  
  Abstract The study of mental workload becomes essential for human work efficiency, health conditions and to avoid accidents, since workload compromises both performance and awareness. Although workload has been widely studied using several physiological measures, minimising the sensor network as much as possible remains both a challenge and a requirement.
Electroencephalogram (EEG) signals have shown a high correlation to specific cognitive and mental states like workload. However, there is not enough evidence in the literature to validate how well models generalize in case of new subjects performing tasks of a workload similar to the ones included during model’s training.
In this paper we propose a binary neural network to classify EEG features across different mental workloads. Two workloads, low and medium, are induced using two variants of the N-Back Test. The proposed model was validated in a dataset collected from 16 subjects and shown a high level of generalization capability: model reported an average recall of 81.81% in a leave-one-out subject evaluation.
 
  Address Virtual; October 20-22 2021  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN ISBN Medium  
  Area Expedition Conference CCIA  
  Notes IAM; 600.139; 600.118; 600.145 Approved no  
  Call Number Admin @ si @ Serial 3723  
Permanent link to this record
 

 
Author Pau Cano; Alvaro Caravaca; Debora Gil; Eva Musulen edit   pdf
url  openurl
  Title Diagnosis of Helicobacter pylori using AutoEncoders for the Detection of Anomalous Staining Patterns in Immunohistochemistry Images Type Miscellaneous
  Year 2023 Publication (up) Arxiv Abbreviated Journal  
  Volume Issue Pages 107241  
  Keywords  
  Abstract This work addresses the detection of Helicobacter pylori a bacterium classified since 1994 as class 1 carcinogen to humans. By its highest specificity and sensitivity, the preferred diagnosis technique is the analysis of histological images with immunohistochemical staining, a process in which certain stained antibodies bind to antigens of the biological element of interest. This analysis is a time demanding task, which is currently done by an expert pathologist that visually inspects the digitized samples.
We propose to use autoencoders to learn latent patterns of healthy tissue and detect H. pylori as an anomaly in image staining. Unlike existing classification approaches, an autoencoder is able to learn patterns in an unsupervised manner (without the need of image annotations) with high performance. In particular, our model has an overall 91% of accuracy with 86\% sensitivity, 96% specificity and 0.97 AUC in the detection of H. pylori.
 
  Address  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN ISBN Medium  
  Area Expedition Conference  
  Notes IAM Approved no  
  Call Number Admin @ si @ CCG2023 Serial 3855  
Permanent link to this record
 

 
Author Debora Gil; Katerine Diaz; Carles Sanchez; Aura Hernandez-Sabate edit   pdf
url  openurl
  Title Early Screening of SARS-CoV-2 by Intelligent Analysis of X-Ray Images Type Miscellaneous
  Year 2020 Publication (up) Arxiv Abbreviated Journal  
  Volume Issue Pages  
  Keywords  
  Abstract Future SARS-CoV-2 virus outbreak COVID-XX might possibly occur during the next years. However the pathology in humans is so recent that many clinical aspects, like early detection of complications, side effects after recovery or early screening, are currently unknown. In spite of the number of cases of COVID-19, its rapid spread putting many sanitary systems in the edge of collapse has hindered proper collection and analysis of the data related to COVID-19 clinical aspects. We describe an interdisciplinary initiative that integrates clinical research, with image diagnostics and the use of new technologies such as artificial intelligence and radiomics with the aim of clarifying some of SARS-CoV-2 open questions. The whole initiative addresses 3 main points: 1) collection of standardize data including images, clinical data and analytics; 2) COVID-19 screening for its early diagnosis at primary care centers; 3) define radiomic signatures of COVID-19 evolution and associated pathologies for the early treatment of complications. In particular, in this paper we present a general overview of the project, the experimental design and first results of X-ray COVID-19 detection using a classic approach based on HoG and feature selection. Our experiments include a comparison to some recent methods for COVID-19 screening in X-Ray and an exploratory analysis of the feasibility of X-Ray COVID-19 screening. Results show that classic approaches can outperform deep-learning methods in this experimental setting, indicate the feasibility of early COVID-19 screening and that non-COVID infiltration is the group of patients most similar to COVID-19 in terms of radiological description of X-ray. Therefore, an efficient COVID-19 screening should be complemented with other clinical data to better discriminate these cases.  
  Address  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN ISBN Medium  
  Area Expedition Conference  
  Notes IAM; 600.139; 600.145; 601.337 Approved no  
  Call Number Admin @ si @ GDS2020 Serial 3474  
Permanent link to this record
 

 
Author Oriol Ramos Terrades; Albert Berenguel; Debora Gil edit   pdf
url  openurl
  Title A flexible outlier detector based on a topology given by graph communities Type Miscellaneous
  Year 2020 Publication (up) Arxiv Abbreviated Journal  
  Volume Issue Pages  
  Keywords  
  Abstract Outlier, or anomaly, detection is essential for optimal performance of machine learning methods and statistical predictive models. It is not just a technical step in a data cleaning process but a key topic in many fields such as fraudulent document detection, in medical applications and assisted diagnosis systems or detecting security threats. In contrast to population-based methods, neighborhood based local approaches are simple flexible methods that have the potential to perform well in small sample size unbalanced problems. However, a main concern of local approaches is the impact that the computation of each sample neighborhood has on the method performance. Most approaches use a distance in the feature space to define a single neighborhood that requires careful selection of several parameters. This work presents a local approach based on a local measure of the heterogeneity of sample labels in the feature space considered as a topological manifold. Topology is computed using the communities of a weighted graph codifying mutual nearest neighbors in the feature space. This way, we provide with a set of multiple neighborhoods able to describe the structure of complex spaces without parameter fine tuning. The extensive experiments on real-world data sets show that our approach overall outperforms, both, local and global strategies in multi and single view settings.  
  Address  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN ISBN Medium  
  Area Expedition Conference  
  Notes IAM; DAG; 600.139; 600.145; 600.140; 600.121 Approved no  
  Call Number Admin @ si @ RBG2020 Serial 3475  
Permanent link to this record
 

 
Author Oriol Ramos Terrades; Albert Berenguel; Debora Gil edit   pdf
doi  openurl
  Title A Flexible Outlier Detector Based on a Topology Given by Graph Communities Type Journal Article
  Year 2022 Publication (up) Big Data Research Abbreviated Journal BDR  
  Volume 29 Issue Pages 100332  
  Keywords Classification algorithms; Detection algorithms; Description of feature space local structure; Graph communities; Machine learning algorithms; Outlier detectors  
  Abstract Outlier detection is essential for optimal performance of machine learning methods and statistical predictive models. Their detection is especially determinant in small sample size unbalanced problems, since in such settings outliers become highly influential and significantly bias models. This particular experimental settings are usual in medical applications, like diagnosis of rare pathologies, outcome of experimental personalized treatments or pandemic emergencies. In contrast to population-based methods, neighborhood based local approaches compute an outlier score from the neighbors of each sample, are simple flexible methods that have the potential to perform well in small sample size unbalanced problems. A main concern of local approaches is the impact that the computation of each sample neighborhood has on the method performance. Most approaches use a distance in the feature space to define a single neighborhood that requires careful selection of several parameters, like the number of neighbors.
This work presents a local approach based on a local measure of the heterogeneity of sample labels in the feature space considered as a topological manifold. Topology is computed using the communities of a weighted graph codifying mutual nearest neighbors in the feature space. This way, we provide with a set of multiple neighborhoods able to describe the structure of complex spaces without parameter fine tuning. The extensive experiments on real-world and synthetic data sets show that our approach outperforms, both, local and global strategies in multi and single view settings.
 
  Address August 28, 2022  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN ISBN Medium  
  Area Expedition Conference  
  Notes DAG; IAM; 600.140; 600.121; 600.139; 600.145; 600.159 Approved no  
  Call Number Admin @ si @ RBG2022a Serial 3718  
Permanent link to this record
 

 
Author Mireia Sole; Joan Blanco; Debora Gil; G. Fonseka; Richard Frodsham; Francesca Vidal; Zaida Sarrate edit   pdf
isbn  openurl
  Title Noves perspectives en l estudi de la territorialitat cromosomica de cel·lules germinals masculines: estudis tridimensionals Type Journal
  Year 2017 Publication (up) Biologia de la Reproduccio Abbreviated Journal JBR  
  Volume 15 Issue Pages 73-78  
  Keywords  
  Abstract In somatic cells, chromosomes occupy specific nuclear regions called chromosome territories which are involved in the
maintenance and regulation of the genome. Preliminary data in male germ cells also suggest the importance of chromosome
territoriality in cell functionality. Nevertheless, the specific characteristics of testicular tissue (presence of different
cell types with different morphological characteristics, in different stages of development and with different ploidy)
makes difficult to achieve conclusive results. In this study we have developed a methodology to approach the threedimensional
study of all chromosome territories in male germ cells from C57BL/6J mice (Mus musculus). The method
includes the following steps: i) Optimized cell fixation to obtain an optimal preservation of the three-dimensionality cell
morphology, ii) Chromosome identification by FISH (Chromoprobe Multiprobe® OctoChrome™ Murine System; Cytocell)
and confocal microscopy (TCS-SP5, Leica Microsystems), iii) Cell type identification by immunofluorescence
iv) Image analysis using Matlab scripts, v) Numerical data extraction related to chromosome features, chromosome
radial position and chromosome relative position. This methodology allows the unequivocally identification and the
analysis of the chromosome territories of all spermatogenic stages. Results will provide information about the features
that determine chromosomal position, preferred associations between chromosomes, and the relationship between chromosome
positioning and genome regulation.
 
  Address  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN ISBN 978-84-697-3767-5 Medium  
  Area Expedition Conference  
  Notes IAM; 600.096; 600.145 Approved no  
  Call Number Admin @ si @ SBG2017c Serial 2961  
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