Abstract: Aggregates of misfolded proteins are a hallmark of many age-related diseases. Recently, they have been linked to aging of Escherichia coli (E. coli) where protein aggregates accumulate at the old pole region of the aging bacterium. Because of the potential of E. coli as a model organism, elucidating aging and protein aggregation in this bacterium may pave the way to significant advances in our global understanding of aging. A first obstacle along this path is to decipher the mechanisms by which protein aggregates are targeted to specific intercellular locations. Here, using an integrated approach based on individual-based modeling, time-lapse fluorescence microscopy and automated image analysis, we show that the movement of aging-related protein aggregates in E. coli is purely diffusive (Brownian). Using single-particle tracking of protein aggregates in live E. coli cells, we estimated the average size and diffusion constant of the aggregates. Our results provide evidence that the aggregates passively diffuse within the cell, with diffusion constants that depend on their size in agreement with the Stokes-Einstein law. However, the aggregate displacements along the cell long axis are confined to a region that roughly corresponds to the nucleoid-free space in the cell pole, thus confirming the importance of increased macromolecular crowding in the nucleoids. We thus used 3D individual-based modeling to show that these three ingredients (diffusion, aggregation and diffusion hindrance in the nucleoids) are sufficient and necessary to reproduce the available experimental data on aggregate localization in the cells. Taken together, our results strongly support the hypothesis that the localization of aging-related protein aggregates in the poles of E. coli results from the coupling of passive diffusion-aggregation with spatially non-homogeneous macromolecular crowding. They further support the importance of “soft” intracellular structuring (based on macromolecular crowding) in diffusion-based protein localization in E. coli.

Abstract: In this paper, we address the problem of symbol spotting in technical document images applied to scanned and vectorized line drawings. Like any information spotting architecture, our approach has two components. First, symbols are decomposed in primitives which are compactly represented and second a primitive indexing structure aims to efficiently retrieve similar primitives. Primitives are encoded in terms of attributed strings representing closed regions. Similar strings are clustered in a lookup table so that the set median strings act as indexing keys. A voting scheme formulates hypothesis in certain locations of the line drawing image where there is a high presence of regions similar to the queried ones, and therefore, a high probability to find the queried graphical symbol. The proposed approach is illustrated in a framework consisting in spotting furniture symbols in architectural drawings. It has been proved to work even in the presence of noise and distortion introduced by the scanning and raster-to-vector processes.

Abstract: Pairwise classification is a well-known class binarization technique that converts a multiclass problem into a number of two-class problems, one problem for each pair of classes. However, in the pairwise technique, nuisance votes of many irrelevant classifiers may result in a wrong class prediction. To overcome this problem, a simple, but efficient method is proposed and evaluated in this paper. The proposed method is based on excluding some classes and focusing on the most probable classes in the neighborhood space, named Local Crossing Off (LCO). This procedure is performed by employing a modified version of standard K-nearest neighbor and large margin nearest neighbor algorithms. The LCO method takes advantage of nearest neighbor classification algorithm because of its local learning behavior as well as the global behavior of powerful binary classifiers to discriminate between two classes. Combining these two properties in the proposed LCO technique will avoid the weaknesses of each method and will increase the efficiency of the whole classification system. On several benchmark datasets of varying size and difficulty, we found that the LCO approach leads to significant improvements using different base learners. The experimental results show that the proposed technique not only achieves better classification accuracy in comparison to other standard approaches, but also is computationally more efficient for tackling classification problems which have a relatively large number of target classes.

Abstract: In this paper, we present a sparse-based denoising algorithm for scanned documents. This method can be applied to any kind of scanned documents with satisfactory results. Unlike other approaches, the proposed approach encodes noise documents through sparse representation and visual dictionary learning techniques without any prior noise model. Moreover, we propose a precision parameter estimator. Experiments on several datasets demonstrate the robustness of the proposed approach compared to the state-of-the-art methods on document denoising.

Abstract: Objectives: In this work we address the need for the computation of quantitative global tumoral state indicators from oncological whole-body PET/computed tomography scans. The combination of such indicators with other oncological information such as tumor markers or biopsy results would prove useful in oncological decision-making scenarios.
Materials and methods: From an ordering of 100 breast cancer patients on the basis of oncological state through visual analysis by a consensus of nuclear medicine specialists, a set of numerical indicators computed from image analysis of the PET/computed tomography scan is presented, which attempts to summarize a patient’s oncological state in a quantitative manner taking into consideration the total tumor volume, aggressiveness, and spread.
Results: Results obtained by comparative analysis of the proposed indicators with respect to the experts’ evaluation show up to 87% Pearson’s correlation coefficient when providing expert-guided PET metabolic tumor volume segmentation and 64% correlation when using completely automatic image analysis techniques.
Conclusion: Global quantitative tumor information obtained by whole-body PET/CT image analysis can prove useful in clinical nuclear medicine settings and oncological decision-making scenarios. The completely automatic computation of such indicators would improve its impact as time efficiency and specialist independence would be achieved.

Abstract: Major advances have recently been made in merging language and vision representations. Most tasks considered so far have confined themselves to the processing of objects and lexicalised relations amongst objects (content words). We know, however, that humans (even pre-school children) can abstract over raw multimodal data to perform certain types of higher level reasoning, expressed in natural language by function words. A case in point is given by their ability to learn quantifiers, i.e. expressions like few, some and all. From formal semantics and cognitive linguistics, we know that quantifiers are relations over sets which, as a simplification, we can see as proportions. For instance, in most fish are red, most encodes the proportion of fish which are red fish. In this paper, we study how well current neural network strategies model such relations. We propose a task where, given an image and a query expressed by an object–property pair, the system must return a quantifier expressing which proportions of the queried object have the queried property. Our contributions are twofold. First, we show that the best performance on this task involves coupling state-of-the-art attention mechanisms with a network architecture mirroring the logical structure assigned to quantifiers by classic linguistic formalisation. Second, we introduce a new balanced dataset of image scenarios associated with quantification queries, which we hope will foster further research in this area.