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Mireia Sole; Joan Blanco; Debora Gil; G. Fonseka; Richard Frodsham; Oliver Valero; Francesca Vidal; Zaida Sarrate |
![download PDF file pdf](http://refbase.cvc.uab.es/img/file_PDF.gif)
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Is there a pattern of Chromosome territoriality along mice spermatogenesis? |
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Conference Article |
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2017 |
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3rd Spanish MeioNet Meeting Abstract Book |
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55-56 |
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Miraflores de la Sierra; Madrid; June 2017 |
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IAM; 600.096; 600.145 |
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2958 |
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Mireia Sole; Joan Blanco; Debora Gil; G. Fonseka; Richard Frodsham; Oliver Valero; Francesca Vidal; Zaida Sarrate |
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Title |
Unraveling the enigmas of chromosome territoriality during spermatogenesis |
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Conference Article |
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2017 |
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IX Jornada del Departament de Biologia Cel•lular, Fisiologia i Immunologia |
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UAB; Barcelona; June 2017 |
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IAM; 600.145 |
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Admin @ si @ SBG2017b |
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2959 |
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Mireia Sole; Joan Blanco; Debora Gil; G. Fonseka; Richard Frodsham; Francesca Vidal; Zaida Sarrate |
![download PDF file pdf](http://refbase.cvc.uab.es/img/file_PDF.gif)
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Title |
Noves perspectives en l estudi de la territorialitat cromosomica de cel·lules germinals masculines: estudis tridimensionals |
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2017 |
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Biologia de la Reproduccio |
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JBR |
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15 |
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73-78 |
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In somatic cells, chromosomes occupy specific nuclear regions called chromosome territories which are involved in the
maintenance and regulation of the genome. Preliminary data in male germ cells also suggest the importance of chromosome
territoriality in cell functionality. Nevertheless, the specific characteristics of testicular tissue (presence of different
cell types with different morphological characteristics, in different stages of development and with different ploidy)
makes difficult to achieve conclusive results. In this study we have developed a methodology to approach the threedimensional
study of all chromosome territories in male germ cells from C57BL/6J mice (Mus musculus). The method
includes the following steps: i) Optimized cell fixation to obtain an optimal preservation of the three-dimensionality cell
morphology, ii) Chromosome identification by FISH (Chromoprobe Multiprobe® OctoChrome™ Murine System; Cytocell)
and confocal microscopy (TCS-SP5, Leica Microsystems), iii) Cell type identification by immunofluorescence
iv) Image analysis using Matlab scripts, v) Numerical data extraction related to chromosome features, chromosome
radial position and chromosome relative position. This methodology allows the unequivocally identification and the
analysis of the chromosome territories of all spermatogenic stages. Results will provide information about the features
that determine chromosomal position, preferred associations between chromosomes, and the relationship between chromosome
positioning and genome regulation. |
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978-84-697-3767-5 |
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IAM; 600.096; 600.145 |
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Admin @ si @ SBG2017c |
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2961 |
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Author |
Pau Cano; Alvaro Caravaca; Debora Gil; Eva Musulen |
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Title |
Diagnosis of Helicobacter pylori using AutoEncoders for the Detection of Anomalous Staining Patterns in Immunohistochemistry Images |
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Miscellaneous |
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2023 |
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Arxiv |
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107241 |
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This work addresses the detection of Helicobacter pylori a bacterium classified since 1994 as class 1 carcinogen to humans. By its highest specificity and sensitivity, the preferred diagnosis technique is the analysis of histological images with immunohistochemical staining, a process in which certain stained antibodies bind to antigens of the biological element of interest. This analysis is a time demanding task, which is currently done by an expert pathologist that visually inspects the digitized samples.
We propose to use autoencoders to learn latent patterns of healthy tissue and detect H. pylori as an anomaly in image staining. Unlike existing classification approaches, an autoencoder is able to learn patterns in an unsupervised manner (without the need of image annotations) with high performance. In particular, our model has an overall 91% of accuracy with 86\% sensitivity, 96% specificity and 0.97 AUC in the detection of H. pylori. |
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Admin @ si @ CCG2023 |
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3855 |
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Author |
Mireia Sole; Joan Blanco; Debora Gil; G. Fonseka; Richard Frodsham; Oliver Valero; Francesca Vidal; Zaida Sarrate |
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Title |
Análisis 3d de la territorialidad cromosómica en células espermatogénicas: explorando la infertilidad desde un nuevo prisma |
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2017 |
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Revista Asociación para el Estudio de la Biología de la Reproducción |
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ASEBIR |
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22 |
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2 |
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105 |
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IAM; 600.096; 600.145 |
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Admin @ si @ SBG2017d |
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3042 |
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Debora Gil; Rosa Maria Ortiz; Carles Sanchez; Antoni Rosell |
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Title |
Objective endoscopic measurements of central airway stenosis. A pilot study |
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Journal Article |
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2018 |
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Respiration |
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RES |
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95 |
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63–69 |
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Bronchoscopy; Tracheal stenosis; Airway stenosis; Computer-assisted analysis |
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Endoscopic estimation of the degree of stenosis in central airway obstruction is subjective and highly variable. Objective: To determine the benefits of using SENSA (System for Endoscopic Stenosis Assessment), an image-based computational software, for obtaining objective stenosis index (SI) measurements among a group of expert bronchoscopists and general pulmonologists. Methods: A total of 7 expert bronchoscopists and 7 general pulmonologists were enrolled to validate SENSA usage. The SI obtained by the physicians and by SENSA were compared with a reference SI to set their precision in SI computation. We used SENSA to efficiently obtain this reference SI in 11 selected cases of benign stenosis. A Web platform with three user-friendly microtasks was designed to gather the data. The users had to visually estimate the SI from videos with and without contours of the normal and the obstructed area provided by SENSA. The users were able to modify the SENSA contours to define the reference SI using morphometric bronchoscopy. Results: Visual SI estimation accuracy was associated with neither bronchoscopic experience (p = 0.71) nor the contours of the normal and the obstructed area provided by the system (p = 0.13). The precision of the SI by SENSA was 97.7% (95% CI: 92.4-103.7), which is significantly better than the precision of the SI by visual estimation (p < 0.001), with an improvement by at least 15%. Conclusion: SENSA provides objective SI measurements with a precision of up to 99.5%, which can be calculated from any bronchoscope using an affordable scalable interface. Providing normal and obstructed contours on bronchoscopic videos does not improve physicians' visual estimation of the SI. |
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IAM; 600.075; 600.096; 600.145 |
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Admin @ si @ GOS2018 |
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3043 |
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Author |
Rosa Maria Ortiz; Debora Gil; Elisa Minchole; Marta Diez-Ferrer; Noelia Cubero de Frutos |
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Title |
Classification of Confolcal Endomicroscopy Patterns for Diagnosis of Lung Cancer |
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Conference Article |
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2017 |
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18th World Conference on Lung Cancer |
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Confocal Laser Endomicroscopy (CLE) is an emerging imaging technique that allows the in-vivo acquisition of cell patterns of potentially malignant lesions. Such patterns could discriminate between inflammatory and neoplastic lesions and, thus, serve as a first in-vivo biopsy to discard cases that do not actually require a cell biopsy.
The goal of this work is to explore whether CLE images obtained during videobronchoscopy contain enough visual information to discriminate between benign and malign peripheral lesions for lung cancer diagnosis. To do so, we have performed a pilot comparative study with 12 patients (6 adenocarcinoma and 6 benign-inflammatory) using 2 different methods for CLE pattern analysis: visual analysis by 3 experts and a novel methodology that uses graph methods to find patterns in pre-trained feature spaces. Our preliminary results indicate that although visual analysis can only achieve a 60.2% of accuracy, the accuracy of the proposed unsupervised image pattern classification raises to 84.6%.
We conclude that CLE images visual information allow in-vivo detection of neoplastic lesions and graph structural analysis applied to deep-learning feature spaces can achieve competitive results. |
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Yokohama; Japan; October 2017 |
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IASLC WCLC |
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IAM; 600.096; 600.075; 600.145 |
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Admin @ si @ OGM2017 |
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3044 |
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Debora Gil; Aura Hernandez-Sabate; David Castells; Jordi Carrabina |
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Title |
CYBERH: Cyber-Physical Systems in Health for Personalized Assistance |
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Conference Article |
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Year |
2017 |
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International Symposium on Symbolic and Numeric Algorithms for Scientific Computing |
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Assistance systems for e-Health applications have some specific requirements that demand of new methods for data gathering, analysis and modeling able to deal with SmallData:
1) systems should dynamically collect data from, both, the environment and the user to issue personalized recommendations; 2) data analysis should be able to tackle a limited number of samples prone to include non-informative data and possibly evolving in time due to changes in patient condition; 3) algorithms should run in real time with possibly limited computational resources and fluctuant internet access.
Electronic medical devices (and CyberPhysical devices in general) can enhance the process of data gathering and analysis in several ways: (i) acquiring simultaneously multiple sensors data instead of single magnitudes (ii) filtering data; (iii) providing real-time implementations condition by isolating tasks in individual processors of multiprocessors Systems-on-chip (MPSoC) platforms and (iv) combining information through sensor fusion
techniques.
Our approach focus on both aspects of the complementary role of CyberPhysical devices and analysis of SmallData in the process of personalized models building for e-Health applications. In particular, we will address the design of Cyber-Physical Systems in Health for Personalized Assistance (CyberHealth) in two specific application cases: 1) A Smart Assisted Driving System (SADs) for dynamical assessment of the driving capabilities of Mild Cognitive Impaired (MCI) people; 2) An Intelligent Operating Room (iOR) for improving the yield of bronchoscopic interventions for in-vivo lung cancer diagnosis. |
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Timisoara; Rumania; September 2017 |
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SYNASC |
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IAM; 600.085; 600.096; 600.075; 600.145 |
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Admin @ si @ GHC2017 |
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3045 |
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Marta Diez-Ferrer; Debora Gil; Elena Carreño; Susana Padrones; Samantha Aso; Vanesa Vicens; Cubero Noelia; Rosa Lopez Lisbona; Carles Sanchez; Agnes Borras; Antoni Rosell |
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Title |
Positive Airway Pressure-Enhanced CT to Improve Virtual Bronchoscopic Navigation |
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Journal Article |
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2016 |
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Chest Journal |
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CHEST |
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150 |
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4 |
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1003A |
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IAM; 600.096; 600.075 |
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Admin @ si @ DGC2016 |
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3099 |
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Esmitt Ramirez; Carles Sanchez; Agnes Borras; Marta Diez-Ferrer; Antoni Rosell; Debora Gil |
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BronchoX: bronchoscopy exploration software for biopsy intervention planning |
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2018 |
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Healthcare Technology Letters |
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HTL |
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5 |
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5 |
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177–182 |
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Virtual bronchoscopy (VB) is a non-invasive exploration tool for intervention planning and navigation of possible pulmonary lesions (PLs). A VB software involves the location of a PL and the calculation of a route, starting from the trachea, to reach it. The selection of a VB software might be a complex process, and there is no consensus in the community of medical software developers in which is the best-suited system to use or framework to choose. The authors present Bronchoscopy Exploration (BronchoX), a VB software to plan biopsy interventions that generate physician-readable instructions to reach the PLs. The authors’ solution is open source, multiplatform, and extensible for future functionalities, designed by their multidisciplinary research and development group. BronchoX is a compound of different algorithms for segmentation, visualisation, and navigation of the respiratory tract. Performed results are a focus on the test the effectiveness of their proposal as an exploration software, also to measure its accuracy as a guiding system to reach PLs. Then, 40 different virtual planning paths were created to guide physicians until distal bronchioles. These results provide a functional software for BronchoX and demonstrate how following simple instructions is possible to reach distal lesions from the trachea. |
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IAM; 600.096; 600.075; 601.323; 601.337; 600.145 |
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Admin @ si @ RSB2018a |
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