Abstract: Quantitative assessment of calcified atherosclerotic volume within the coronary artery wall is vital for cardiac interventional procedures. The goal of this study is to automatically measure the calcium volume, given the borders of coronary vessel wall for all the frames of the intravascular ultrasound (IVUS) video. Three soft computing fuzzy classification techniques were adapted namely Fuzzy c-Means (FCM), K-means, and Hidden Markov Random Field (HMRF) for automated segmentation of calcium regions and volume computation. These methods were benchmarked against previously developed threshold-based method. IVUS image data sets (around 30,600 IVUS frames) from 15 patients were collected using 40 MHz IVUS catheter (Atlantis® SR Pro, Boston Scientific®, pullback speed of 0.5 mm/s). Calcium mean volume for FCM, K-means, HMRF and threshold-based method were 37.84 ± 17.38 mm3, 27.79 ± 10.94 mm3, 46.44 ± 19.13 mm3 and 35.92 ± 16.44 mm3 respectively. Cross-correlation, Jaccard Index and Dice Similarity were highest between FCM and threshold-based method: 0.99, 0.92 ± 0.02 and 0.95 + 0.02 respectively. Student’s t-test, z-test and Wilcoxon-test are also performed to demonstrate consistency, reliability and accuracy of the results. Given the vessel wall region, the system reliably and automatically measures the calcium volume in IVUS videos. Further, we validated our system against a trained expert using scoring: K-means showed the best performance with an accuracy of 92.80 %. Out procedure and protocol is along the line with method previously published clinically.

Abstract: Wearable cameras offer a hands-free way to record egocentric images of daily experiences, where social events are of special interest. The first step towards detection of social events is to track the appearance of multiple persons involved in them. In this paper, we propose a novel method to find correspondences of multiple faces in low temporal resolution egocentric videos acquired through a wearable camera. This kind of photo-stream imposes additional challenges to the multi-tracking problem with respect to conventional videos. Due to the free motion of the camera and to its low temporal resolution, abrupt changes in the field of view, in illumination condition and in the target location are highly frequent. To overcome such difficulties, we propose a multi-face tracking method that generates a set of tracklets through finding correspondences along the whole sequence for each detected face and takes advantage of the tracklets redundancy to deal with unreliable ones. Similar tracklets are grouped into the so called extended bag-of-tracklets (eBoT), which is aimed to correspond to a specific person. Finally, a prototype tracklet is extracted for each eBoT, where the occurred occlusions are estimated by relying on a new measure of confidence. We validated our approach over an extensive dataset of egocentric photo-streams and compared it to state of the art methods, demonstrating its effectiveness and robustness.

Abstract: The investigation of spatio-temporal dynamics of bacterial cells and their molecular components requires automated image analysis tools to track cell shape properties and molecular component locations inside the cells. In the study of bacteria aging, the molecular components of interest are protein aggregates accumulated near bacteria boundaries. This particular location makes very ambiguous the correspondence between aggregates and cells, since computing accurately bacteria boundaries in phase-contrast time-lapse imaging is a challenging task. This paper proposes an active skeleton formulation for bacteria modelling which provides several advantages: an easy computation of shape properties (perimeter, length, thickness and orientation), an improved boundary accuracy in noisy images and a natural bacteria-centred coordinate system that permits the intrinsic location of molecular components inside the cell. Starting from an initial skeleton estimate, the medial axis of the bacterium is obtained by minimising an energy function which incorporates bacteria shape constraints. Experimental results on biological images and comparative evaluation of the performances validate the proposed approach for modelling cigar-shaped bacteria like Escherichia coli. The Image-J plugin of the proposed method can be found online at http://fluobactracker.inrialpes.fr.