PT Journal AU Mireia Sole Joan Blanco Debora Gil G. Fonseka Richard Frodsham Francesca Vidal Zaida Sarrate TI Noves perspectives en l estudi de la territorialitat cromosomica de cel·lules germinals masculines: estudis tridimensionals PY 2017 AB In somatic cells, chromosomes occupy specific nuclear regions called chromosome territories which are involved in themaintenance and regulation of the genome. Preliminary data in male germ cells also suggest the importance of chromosometerritoriality in cell functionality. Nevertheless, the specific characteristics of testicular tissue (presence of differentcell types with different morphological characteristics, in different stages of development and with different ploidy)makes difficult to achieve conclusive results. In this study we have developed a methodology to approach the threedimensionalstudy of all chromosome territories in male germ cells from C57BL/6J mice (Mus musculus). The methodincludes the following steps: i) Optimized cell fixation to obtain an optimal preservation of the three-dimensionality cellmorphology, ii) Chromosome identification by FISH (Chromoprobe Multiprobe® OctoChrome™ Murine System; Cytocell)and confocal microscopy (TCS-SP5, Leica Microsystems), iii) Cell type identification by immunofluorescenceiv) Image analysis using Matlab scripts, v) Numerical data extraction related to chromosome features, chromosomeradial position and chromosome relative position. This methodology allows the unequivocally identification and theanalysis of the chromosome territories of all spermatogenic stages. Results will provide information about the featuresthat determine chromosomal position, preferred associations between chromosomes, and the relationship between chromosomepositioning and genome regulation. ER