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Mireia Sole; Joan Blanco; Debora Gil; Oliver Valero; B. Cardenas; G. Fonseka; E. Anton; Alvaro Pascual; Richard Frodsham; Zaida Sarrate |
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Time to match; when do homologous chromosomes become closer? |
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2022 |
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Chromosoma |
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CHRO |
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In most eukaryotes, pairing of homologous chromosomes is an essential feature of meiosis that ensures homologous recombination and segregation. However, when the pairing process begins, it is still under investigation. Contrasting data exists in Mus musculus, since both leptotene DSB-dependent and preleptotene DSB-independent mechanisms have been described. To unravel this contention, we examined homologous pairing in pre-meiotic and meiotic Mus musculus cells using a threedimensional fuorescence in situ hybridization-based protocol, which enables the analysis of the entire karyotype using DNA painting probes. Our data establishes in an unambiguously manner that 73.83% of homologous chromosomes are already paired at premeiotic stages (spermatogonia-early preleptotene spermatocytes). The percentage of paired homologous chromosomes increases to 84.60% at mid-preleptotene-zygotene stage, reaching 100% at pachytene stage. Importantly, our results demonstrate a high percentage of homologous pairing observed before the onset of meiosis; this pairing does not occur randomly, as the percentage was higher than that observed in somatic cells (19.47%) and between nonhomologous chromosomes (41.1%). Finally, we have also observed that premeiotic homologous pairing is asynchronous and independent of the chromosome size, GC content, or presence of NOR regions. |
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August, 2022 |
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IAM; 601.139; 600.145; 600.096 |
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Admin @ si @ SBG2022 |
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3719 |
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Guillermo Torres; Debora Gil; Antoni Rosell; S. Mena; Carles Sanchez |
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Virtual Radiomics Biopsy for the Histological Diagnosis of Pulmonary Nodules – Intermediate Results of the RadioLung Project |
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2023 |
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International Journal of Computer Assisted Radiology and Surgery |
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IJCARS |
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Admin @ si @ TGM2023 |
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3830 |
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Juan Borrego-Carazo; Carles Sanchez; David Castells; Jordi Carrabina; Debora Gil |
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A benchmark for the evaluation of computational methods for bronchoscopic navigation |
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2022 |
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International Journal of Computer Assisted Radiology and Surgery |
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IJCARS |
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17 |
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1 |
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Admin @ si @ BSC2022 |
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3832 |
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Antoni Rosell; Sonia Baeza; S. Garcia-Reina; JL. Mate; Ignasi Guasch; I. Nogueira; I. Garcia-Olive; Guillermo Torres; Carles Sanchez; Debora Gil |
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Radiomics to increase the effectiveness of lung cancer screening programs. Radiolung preliminary results. |
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2022 |
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European Respiratory Journal |
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ERJ |
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60 |
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66 |
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Admin @ si @ RBG2022c |
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3835 |
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