| |
Records |
Links |
|
Author |
Carles Sanchez; Jorge Bernal; F. Javier Sanchez; Marta Diez-Ferrer; Antoni Rosell; Debora Gil |
|
| |
Title |
Towards On-line Quantification of Tracheal Stenosis from Videobronchoscopy |
Type |
Conference Article |
| |
Year |
2015 |
Publication |
6th International Conference on Information Processing in Computer-Assisted Interventions IPCAI2015 |
Abbreviated Journal |
|
|
| |
Volume |
10 |
Issue |
6 |
Pages |
935-945 |
|
| |
Keywords |
|
|
| |
Abstract |
PURPOSE:
Lack of objective measurement of tracheal obstruction degree has a negative impact on the chosen treatment prone to lead to unnecessary repeated explorations and other scanners. Accurate computation of tracheal stenosis in videobronchoscopy would constitute a breakthrough for this noninvasive technique and a reduction in operation cost for the public health service.
METHODS:
Stenosis calculation is based on the comparison of the region delimited by the lumen in an obstructed frame and the region delimited by the first visible ring in a healthy frame. We propose a parametric strategy for the extraction of lumen and tracheal ring regions based on models of their geometry and appearance that guide a deformable model. To ensure a systematic applicability, we present a statistical framework to choose optimal parametric values and a strategy to choose the frames that minimize the impact of scope optical distortion.
RESULTS:
Our method has been tested in 40 cases covering different stenosed tracheas. Experiments report a non- clinically relevant [Formula: see text] of discrepancy in the calculated stenotic area and a computational time allowing online implementation in the operating room.
CONCLUSIONS:
Our methodology allows reliable measurements of airway narrowing in the operating room. To fully assess its clinical impact, a prospective clinical trial should be done. |
|
| |
Address |
Barcelona; Spain; June 2015 |
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
|
Place of Publication |
|
Editor |
|
|
| |
Language |
|
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
|
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
IPCAI |
|
| |
Notes |
IAM; MV; 600.075 |
Approved |
no |
|
| |
Call Number  |
Admin @ si @ SBS2015b |
Serial |
2613 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Carles Sanchez; Jorge Bernal; F. Javier Sanchez; Antoni Rosell; Marta Diez-Ferrer; Debora Gil |
|
| |
Title |
Towards On-line Quantification of Tracheal Stenosis from Videobronchoscopy |
Type |
Journal Article |
| |
Year |
2015 |
Publication |
International Journal of Computer Assisted Radiology and Surgery |
Abbreviated Journal |
IJCAR |
|
| |
Volume |
10 |
Issue |
6 |
Pages |
935-945 |
|
| |
Keywords |
|
|
| |
Abstract |
|
|
| |
Address |
|
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
|
Place of Publication |
|
Editor |
|
|
| |
Language |
|
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
|
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
IAM; MV; 600.075 |
Approved |
no |
|
| |
Call Number |
Admin @ si @ SBS2015a |
Serial |
2611 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Mireia Sole; Joan Blanco; Debora Gil; Oliver Valero; B. Cardenas; G. Fonseka; E. Anton; Alvaro Pascual; Richard Frodsham; Zaida Sarrate |
|
| |
Title |
Time to match; when do homologous chromosomes become closer? |
Type |
Journal Article |
| |
Year |
2022 |
Publication |
Chromosoma |
Abbreviated Journal |
CHRO |
|
| |
Volume |
|
Issue |
|
Pages |
|
|
| |
Keywords |
|
|
| |
Abstract |
In most eukaryotes, pairing of homologous chromosomes is an essential feature of meiosis that ensures homologous recombination and segregation. However, when the pairing process begins, it is still under investigation. Contrasting data exists in Mus musculus, since both leptotene DSB-dependent and preleptotene DSB-independent mechanisms have been described. To unravel this contention, we examined homologous pairing in pre-meiotic and meiotic Mus musculus cells using a threedimensional fuorescence in situ hybridization-based protocol, which enables the analysis of the entire karyotype using DNA painting probes. Our data establishes in an unambiguously manner that 73.83% of homologous chromosomes are already paired at premeiotic stages (spermatogonia-early preleptotene spermatocytes). The percentage of paired homologous chromosomes increases to 84.60% at mid-preleptotene-zygotene stage, reaching 100% at pachytene stage. Importantly, our results demonstrate a high percentage of homologous pairing observed before the onset of meiosis; this pairing does not occur randomly, as the percentage was higher than that observed in somatic cells (19.47%) and between nonhomologous chromosomes (41.1%). Finally, we have also observed that premeiotic homologous pairing is asynchronous and independent of the chromosome size, GC content, or presence of NOR regions. |
|
| |
Address |
August, 2022 |
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
|
Place of Publication |
|
Editor |
|
|
| |
Language |
|
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
|
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
IAM; 601.139; 600.145; 600.096 |
Approved |
no |
|
| |
Call Number |
Admin @ si @ SBG2022 |
Serial |
3719 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Mireia Sole; Joan Blanco; Debora Gil; Oliver Valero; Alvaro Pascual; B. Cardenas; G. Fonseka; E. Anton; Richard Frodsham; Francesca Vidal; Zaida Sarrate |
|
| |
Title |
Chromosomal positioning in spermatogenic cells is influenced by chromosomal factors associated with gene activity, bouquet formation, and meiotic sex-chromosome inactivation |
Type |
Journal Article |
| |
Year |
2021 |
Publication |
Chromosoma |
Abbreviated Journal |
|
|
| |
Volume |
130 |
Issue |
|
Pages |
163-175 |
|
| |
Keywords |
|
|
| |
Abstract |
Chromosome territoriality is not random along the cell cycle and it is mainly governed by intrinsic chromosome factors and gene expression patterns. Conversely, very few studies have explored the factors that determine chromosome territoriality and its influencing factors during meiosis. In this study, we analysed chromosome positioning in murine spermatogenic cells using three-dimensionally fluorescence in situ hybridization-based methodology, which allows the analysis of the entire karyotype. The main objective of the study was to decipher chromosome positioning in a radial axis (all analysed germ-cell nuclei) and longitudinal axis (only spermatozoa) and to identify the chromosomal factors that regulate such an arrangement. Results demonstrated that the radial positioning of chromosomes during spermatogenesis was cell-type specific and influenced by chromosomal factors associated to gene activity. Chromosomes with specific features that enhance transcription (high GC content, high gene density and high numbers of predicted expressed genes) were preferentially observed in the inner part of the nucleus in virtually all cell types. Moreover, the position of the sex chromosomes was influenced by their transcriptional status, from the periphery of the nucleus when its activity was repressed (pachytene) to a more internal position when it is partially activated (spermatid). At pachytene, chromosome positioning was also influenced by chromosome size due to the bouquet formation. Longitudinal chromosome positioning in the sperm nucleus was not random either, suggesting the importance of ordered longitudinal positioning for the release and activation of the paternal genome after fertilisation. |
|
| |
Address |
|
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
|
Place of Publication |
|
Editor |
|
|
| |
Language |
|
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
|
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
IAM; 600.145 |
Approved |
no |
|
| |
Call Number |
Admin @ si @ SBG2021 |
Serial |
3592 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Mireia Sole; Joan Blanco; Debora Gil; G. Fonseka; Richard Frodsham; Oliver Valero; Francesca Vidal; Zaida Sarrate |
|
| |
Title |
Análisis 3d de la territorialidad cromosómica en células espermatogénicas: explorando la infertilidad desde un nuevo prisma |
Type |
Journal |
| |
Year |
2017 |
Publication |
Revista Asociación para el Estudio de la Biología de la Reproducción |
Abbreviated Journal |
ASEBIR |
|
| |
Volume |
22 |
Issue |
2 |
Pages |
105 |
|
| |
Keywords |
|
|
| |
Abstract |
|
|
| |
Address |
|
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
|
Place of Publication |
|
Editor |
|
|
| |
Language |
|
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
|
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
IAM; 600.096; 600.145 |
Approved |
no |
|
| |
Call Number |
Admin @ si @ SBG2017d |
Serial |
3042 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Mireia Sole; Joan Blanco; Debora Gil; G. Fonseka; Richard Frodsham; Francesca Vidal; Zaida Sarrate |
|
| |
Title |
Noves perspectives en l estudi de la territorialitat cromosomica de cel·lules germinals masculines: estudis tridimensionals |
Type |
Journal |
| |
Year |
2017 |
Publication |
Biologia de la Reproduccio |
Abbreviated Journal |
JBR |
|
| |
Volume |
15 |
Issue |
|
Pages |
73-78 |
|
| |
Keywords |
|
|
| |
Abstract |
In somatic cells, chromosomes occupy specific nuclear regions called chromosome territories which are involved in the
maintenance and regulation of the genome. Preliminary data in male germ cells also suggest the importance of chromosome
territoriality in cell functionality. Nevertheless, the specific characteristics of testicular tissue (presence of different
cell types with different morphological characteristics, in different stages of development and with different ploidy)
makes difficult to achieve conclusive results. In this study we have developed a methodology to approach the threedimensional
study of all chromosome territories in male germ cells from C57BL/6J mice (Mus musculus). The method
includes the following steps: i) Optimized cell fixation to obtain an optimal preservation of the three-dimensionality cell
morphology, ii) Chromosome identification by FISH (Chromoprobe Multiprobe® OctoChrome™ Murine System; Cytocell)
and confocal microscopy (TCS-SP5, Leica Microsystems), iii) Cell type identification by immunofluorescence
iv) Image analysis using Matlab scripts, v) Numerical data extraction related to chromosome features, chromosome
radial position and chromosome relative position. This methodology allows the unequivocally identification and the
analysis of the chromosome territories of all spermatogenic stages. Results will provide information about the features
that determine chromosomal position, preferred associations between chromosomes, and the relationship between chromosome
positioning and genome regulation. |
|
| |
Address |
|
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
|
Place of Publication |
|
Editor |
|
|
| |
Language |
|
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
|
ISBN |
978-84-697-3767-5 |
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
IAM; 600.096; 600.145 |
Approved |
no |
|
| |
Call Number |
Admin @ si @ SBG2017c |
Serial |
2961 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Mireia Sole; Joan Blanco; Debora Gil; G. Fonseka; Richard Frodsham; Oliver Valero; Francesca Vidal; Zaida Sarrate |
|
| |
Title |
Unraveling the enigmas of chromosome territoriality during spermatogenesis |
Type |
Conference Article |
| |
Year |
2017 |
Publication |
IX Jornada del Departament de Biologia Cel•lular, Fisiologia i Immunologia |
Abbreviated Journal |
|
|
| |
Volume |
|
Issue |
|
Pages |
|
|
| |
Keywords |
|
|
| |
Abstract |
|
|
| |
Address |
UAB; Barcelona; June 2017 |
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
|
Place of Publication |
|
Editor |
|
|
| |
Language |
|
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
|
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
IAM; 600.145 |
Approved |
no |
|
| |
Call Number |
Admin @ si @ SBG2017b |
Serial |
2959 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Mireia Sole; Joan Blanco; Debora Gil; Oliver Valero; G. Fonseka; M. Lawrie; Francesca Vidal; Zaida Sarrate |
|
| |
Title |
Chromosome Territories in Mice Spermatogenesis: A new three-dimensional methodology of study |
Type |
Conference Article |
| |
Year |
2017 |
Publication |
11th European CytoGenesis Conference |
Abbreviated Journal |
|
|
| |
Volume |
|
Issue |
|
Pages |
|
|
| |
Keywords |
|
|
| |
Abstract |
|
|
| |
Address |
Florencia; Italia; July 2017 |
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
|
Place of Publication |
|
Editor |
|
|
| |
Language |
|
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
|
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
ECA |
|
| |
Notes |
IAM; 600.096; 600.145 |
Approved |
no |
|
| |
Call Number |
Admin @ si @ SBG2017a |
Serial |
2936 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Carles Sanchez; Jorge Bernal; Debora Gil; F. Javier Sanchez |
|
| |
Title |
On-line lumen centre detection in gastrointestinal and respiratory endoscopy |
Type |
Conference Article |
| |
Year |
2013 |
Publication |
Second International Workshop Clinical Image-Based Procedures |
Abbreviated Journal |
|
|
| |
Volume |
8361 |
Issue |
|
Pages |
31-38 |
|
| |
Keywords |
Lumen centre detection; Bronchoscopy; Colonoscopy |
|
| |
Abstract |
We present in this paper a novel lumen centre detection for gastrointestinal and respiratory endoscopic images. The proposed method is based on the appearance and geometry of the lumen, which we defined as the darkest image region which centre is a hub of image gradients. Experimental results validated on the first public annotated gastro-respiratory database prove the reliability of the method for a wide range of images (with precision over 95 %). |
|
| |
Address |
Nagoya; Japan; September 2013 |
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
Springer International Publishing |
Place of Publication |
|
Editor |
Erdt, Marius and Linguraru, Marius George and Oyarzun Laura, Cristina and Shekhar, Raj and Wesarg, Stefan and González Ballester, Miguel Angel and Drechsler, Klaus |
|
| |
Language |
|
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
LNCS |
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
|
ISBN |
978-3-319-05665-4 |
Medium |
|
|
| |
Area |
800 |
Expedition |
|
Conference |
CLIP |
|
| |
Notes |
MV; IAM; 600.047; 600.044; 600.060 |
Approved |
no |
|
| |
Call Number |
Admin @ si @ SBG2013 |
Serial |
2302 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Carles Sanchez |
|
| |
Title |
Tracheal Structure Characterization using Geometric and Appearance Models for Efficient Assessment of Stenosis in Videobronchoscopy |
Type |
Book Whole |
| |
Year |
2014 |
Publication |
PhD Thesis, Universitat Autonoma de Barcelona-CVC |
Abbreviated Journal |
|
|
| |
Volume |
|
Issue |
|
Pages |
|
|
| |
Keywords |
|
|
| |
Abstract |
Recent advances in endoscopic devices have increased their use for minimal invasive diagnostic and intervention procedures. Among all endoscopic modalities, bronchoscopy is one of the most frequent with around 261 millions of procedures per year. Although the use of bronchoscopy is spread among clinical facilities it presents some drawbacks, being the visual inspection for the assessment of anatomical measurements the most prevalent of them. In
particular, inaccuracies in the estimation of the degree of stenosis (the percentage of obstructed airway) decreases its diagnostic yield and might lead to erroneous treatments. An objective computation of tracheal stenosis in bronchoscopy videos would constitute a breakthrough for this non-invasive technique and a reduction in treatment cost.
This thesis settles the first steps towards on-line reliable extraction of anatomical information from videobronchoscopy for computation of objective measures. In particular, we focus on the computation of the degree of stenosis, which is obtained by comparing the area delimited by a healthy tracheal ring and the stenosed lumen. Reliable extraction of airway structures in interventional videobronchoscopy is a challenging task. This is mainly due to the large variety of acquisition conditions (positions and illumination), devices (different digitalizations) and in videos acquired at the operating room the unpredicted presence of surgical devices (such as probe ends). This thesis contributes to on-line stenosis assessment in several ways. We
propose a parametric strategy for the extraction of lumen and tracheal rings regions based on the characterization of their geometry and appearance that guide a deformable model. The geometric and appearance characterization is based on a physical model describing the way bronchoscopy images are obtained and includes local and global descriptions. In order to ensure a systematic applicability we present a statistical framework to select the optimal
parameters of our method. Experiments perform on the first public annotated database, show that the performance of our method is comparable to the one provided by clinicians and its computation time allows for a on-line implementation in the operating room. |
|
| |
Address |
|
|
| |
Corporate Author |
|
Thesis |
Ph.D. thesis |
|
| |
Publisher |
Ediciones Graficas Rey |
Place of Publication |
|
Editor |
F. Javier Sanchez;Debora Gil;Jorge Bernal |
|
| |
Language |
|
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
|
ISBN |
978-84-940902-9-5 |
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
IAM; 600.075 |
Approved |
no |
|
| |
Call Number |
Admin @ si @ San2014 |
Serial |
2575 |
|
| Permanent link to this record |
