Abstract: Comic books digitization combined with subsequent comic book understanding create a variety of new applications, including mobile reading and data mining. Document understanding in this domain is challenging as comics are semi-structured documents, combining semantically important graphical and textual parts. In this work we detail a novel approach for classifying speech balloon in scanned comics book pages based on their contour time series.

Abstract: Motion perception is one of the most important attributes of the human brain. Visual motion perception consists in inferring speed and direction of elements in a scene based on visual inputs. Analogously, computer vision is assisted by motion cues in the scene. Motion detection in computer vision is useful in solving problems such as segmentation, depth from motion, structure from motion, compression, navigation and many others. These problems are common in several applications, for instance, video surveillance, robot navigation and advanced driver assistance systems (ADAS). One of the most widely used techniques for motion detection is the optical flow estimation. The work in this thesis attempts to make optical flow suitable for the requirements and conditions of driving scenarios. In this context, a novel space-variant representation called reverse log-polar representation is proposed that is shown to be better than the traditional log-polar space-variant representation for ADAS. The space-variant representations reduce the amount of data to be processed. Another major contribution in this research is related to the analysis of the influence of specific characteristics from driving scenarios on the optical flow accuracy. Characteristics such as vehicle speed and
road texture are considered in the aforementioned analysis. From this study, it is inferred that the regularization weight has to be adapted according to the required error measure and for different speeds and road textures. It is also shown that polar represented optical flow suits driving scenarios where predominant motion is translation. Due to the requirements of such a study and by the lack of needed datasets a new synthetic dataset is presented; it contains: i) sequences of different speeds and road textures in an urban scenario; ii) sequences with complex motion of an on-board camera; and iii) sequences with additional moving vehicles in the scene. The ground-truth optical flow is generated by the ray-tracing technique. Further, few applications of optical flow in ADAS are shown. Firstly, a robust RANSAC based technique to estimate horizon line is proposed. Then, an egomotion estimation is presented to compare the proposed space-variant representation with the classical one. As a final contribution, a modification in the regularization term is proposed that notably improves the results
in the ADAS applications. This adaptation is evaluated using a state of the art optical flow technique. The experiments on a public dataset (KITTI) validate the advantages of using the proposed modification.

Abstract: Aggregates of misfolded proteins are a hallmark of many age-related diseases. Recently, they have been linked to aging of Escherichia coli (E. coli) where protein aggregates accumulate at the old pole region of the aging bacterium. Because of the potential of E. coli as a model organism, elucidating aging and protein aggregation in this bacterium may pave the way to significant advances in our global understanding of aging. A first obstacle along this path is to decipher the mechanisms by which protein aggregates are targeted to specific intercellular locations. Here, using an integrated approach based on individual-based modeling, time-lapse fluorescence microscopy and automated image analysis, we show that the movement of aging-related protein aggregates in E. coli is purely diffusive (Brownian). Using single-particle tracking of protein aggregates in live E. coli cells, we estimated the average size and diffusion constant of the aggregates. Our results provide evidence that the aggregates passively diffuse within the cell, with diffusion constants that depend on their size in agreement with the Stokes-Einstein law. However, the aggregate displacements along the cell long axis are confined to a region that roughly corresponds to the nucleoid-free space in the cell pole, thus confirming the importance of increased macromolecular crowding in the nucleoids. We thus used 3D individual-based modeling to show that these three ingredients (diffusion, aggregation and diffusion hindrance in the nucleoids) are sufficient and necessary to reproduce the available experimental data on aggregate localization in the cells. Taken together, our results strongly support the hypothesis that the localization of aging-related protein aggregates in the poles of E. coli results from the coupling of passive diffusion-aggregation with spatially non-homogeneous macromolecular crowding. They further support the importance of “soft” intracellular structuring (based on macromolecular crowding) in diffusion-based protein localization in E. coli.