|
Records |
Links |
|
Author |
Carles Sanchez; Jorge Bernal; F. Javier Sanchez; Marta Diez-Ferrer; Antoni Rosell; Debora Gil |
![find record details (via OpenURL) openurl](img/xref.gif)
|
|
Title |
Towards On-line Quantification of Tracheal Stenosis from Videobronchoscopy |
Type |
Conference Article |
|
Year |
2015 |
Publication |
6th International Conference on Information Processing in Computer-Assisted Interventions IPCAI2015 |
Abbreviated Journal |
|
|
|
Volume |
10 |
Issue |
6 |
Pages |
935-945 |
|
|
Keywords |
|
|
|
Abstract |
PURPOSE:
Lack of objective measurement of tracheal obstruction degree has a negative impact on the chosen treatment prone to lead to unnecessary repeated explorations and other scanners. Accurate computation of tracheal stenosis in videobronchoscopy would constitute a breakthrough for this noninvasive technique and a reduction in operation cost for the public health service.
METHODS:
Stenosis calculation is based on the comparison of the region delimited by the lumen in an obstructed frame and the region delimited by the first visible ring in a healthy frame. We propose a parametric strategy for the extraction of lumen and tracheal ring regions based on models of their geometry and appearance that guide a deformable model. To ensure a systematic applicability, we present a statistical framework to choose optimal parametric values and a strategy to choose the frames that minimize the impact of scope optical distortion.
RESULTS:
Our method has been tested in 40 cases covering different stenosed tracheas. Experiments report a non- clinically relevant [Formula: see text] of discrepancy in the calculated stenotic area and a computational time allowing online implementation in the operating room.
CONCLUSIONS:
Our methodology allows reliable measurements of airway narrowing in the operating room. To fully assess its clinical impact, a prospective clinical trial should be done. |
|
|
Address |
Barcelona; Spain; June 2015 |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
|
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
|
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
IPCAI |
|
|
Notes |
IAM; MV; 600.075 |
Approved |
no |
|
|
Call Number ![sorted by Call Number field, descending order (down)](img/sort_desc.gif) |
Admin @ si @ SBS2015b |
Serial |
2613 |
|
Permanent link to this record |
|
|
|
|
Author |
Carles Sanchez; Jorge Bernal; F. Javier Sanchez; Antoni Rosell; Marta Diez-Ferrer; Debora Gil |
![download PDF file pdf](img/file_PDF.gif)
![find record details (via OpenURL) openurl](img/xref.gif)
|
|
Title |
Towards On-line Quantification of Tracheal Stenosis from Videobronchoscopy |
Type |
Journal Article |
|
Year |
2015 |
Publication |
International Journal of Computer Assisted Radiology and Surgery |
Abbreviated Journal |
IJCAR |
|
|
Volume |
10 |
Issue |
6 |
Pages |
935-945 |
|
|
Keywords |
|
|
|
Abstract |
|
|
|
Address |
|
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
|
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
|
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
IAM; MV; 600.075 |
Approved |
no |
|
|
Call Number ![sorted by Call Number field, descending order (down)](img/sort_desc.gif) |
Admin @ si @ SBS2015a |
Serial |
2611 |
|
Permanent link to this record |
|
|
|
|
Author |
C. Sbert; A.F. Sole |
![find record details (via OpenURL) openurl](img/xref.gif)
|
|
Title |
Stereo reconstruction of 3D curves. |
Type |
Conference Article |
|
Year |
2000 |
Publication |
15 th International Conference on Pattern Recognition |
Abbreviated Journal |
|
|
|
Volume |
1 |
Issue |
|
Pages |
912–915 |
|
|
Keywords |
|
|
|
Abstract |
|
|
|
Address |
Barcelona. |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
|
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
|
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
ICPR |
|
|
Notes |
|
Approved |
no |
|
|
Call Number ![sorted by Call Number field, descending order (down)](img/sort_desc.gif) |
Admin @ si @ SbS2000 |
Serial |
219 |
|
Permanent link to this record |
|
|
|
|
Author |
Md. Mostafa Kamal Sarker; Syeda Furruka Banu; Hatem A. Rashwan; Mohamed Abdel-Nasser; Vivek Kumar Singh; Sylvie Chambon; Petia Radeva; Domenec Puig |
![goto web page (via DOI) doi](img/doi.gif)
|
|
Title |
Food Places Classification in Egocentric Images Using Siamese Neural Networks |
Type |
Conference Article |
|
Year |
2019 |
Publication |
22nd International Conference of the Catalan Association of Artificial Intelligence |
Abbreviated Journal |
|
|
|
Volume |
|
Issue |
|
Pages |
145-151 |
|
|
Keywords |
|
|
|
Abstract |
Wearable cameras are become more popular in recent years for capturing the unscripted moments of the first-person that help to analyze the users lifestyle. In this work, we aim to recognize the places related to food in egocentric images during a day to identify the daily food patterns of the first-person. Thus, this system can assist to improve their eating behavior to protect users against food-related diseases. In this paper, we use Siamese Neural Networks to learn the similarity between images from corresponding inputs for one-shot food places classification. We tested our proposed method with ‘MiniEgoFoodPlaces’ with 15 food related places. The proposed Siamese Neural Networks model with MobileNet achieved an overall classification accuracy of 76.74% and 77.53% on the validation and test sets of the “MiniEgoFoodPlaces” dataset, respectively outperforming with the base models, such as ResNet50, InceptionV3, and InceptionResNetV2. |
|
|
Address |
Illes Balears; October 2019 |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
|
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
|
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
CCIA |
|
|
Notes |
MILAB; no proj |
Approved |
no |
|
|
Call Number ![sorted by Call Number field, descending order (down)](img/sort_desc.gif) |
Admin @ si @ SBR2019 |
Serial |
3368 |
|
Permanent link to this record |
|
|
|
|
Author |
Mohamed Ali Souibgui; Sanket Biswas; Andres Mafla; Ali Furkan Biten; Alicia Fornes; Yousri Kessentini; Josep Llados; Lluis Gomez; Dimosthenis Karatzas |
![goto web page url](img/www.gif)
|
|
Title |
Text-DIAE: a self-supervised degradation invariant autoencoder for text recognition and document enhancement |
Type |
Conference Article |
|
Year |
2023 |
Publication |
Proceedings of the 37th AAAI Conference on Artificial Intelligence |
Abbreviated Journal |
|
|
|
Volume |
37 |
Issue |
2 |
Pages |
|
|
|
Keywords |
Representation Learning for Vision; CV Applications; CV Language and Vision; ML Unsupervised; Self-Supervised Learning |
|
|
Abstract |
In this paper, we propose a Text-Degradation Invariant Auto Encoder (Text-DIAE), a self-supervised model designed to tackle two tasks, text recognition (handwritten or scene-text) and document image enhancement. We start by employing a transformer-based architecture that incorporates three pretext tasks as learning objectives to be optimized during pre-training without the usage of labelled data. Each of the pretext objectives is specifically tailored for the final downstream tasks. We conduct several ablation experiments that confirm the design choice of the selected pretext tasks. Importantly, the proposed model does not exhibit limitations of previous state-of-the-art methods based on contrastive losses, while at the same time requiring substantially fewer data samples to converge. Finally, we demonstrate that our method surpasses the state-of-the-art in existing supervised and self-supervised settings in handwritten and scene text recognition and document image enhancement. Our code and trained models will be made publicly available at https://github.com/dali92002/SSL-OCR |
|
|
Address |
|
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
|
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
|
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
AAAI |
|
|
Notes |
DAG |
Approved |
no |
|
|
Call Number ![sorted by Call Number field, descending order (down)](img/sort_desc.gif) |
Admin @ si @ SBM2023 |
Serial |
3848 |
|
Permanent link to this record |
|
|
|
|
Author |
J.M. Sanchez; X. Binefa; J.R. Kender |
![find record details (via OpenURL) openurl](img/xref.gif)
|
|
Title |
Multiple Feature Temporal Models for Object Detection in Video. |
Type |
Miscellaneous |
|
Year |
2002 |
Publication |
Proceeding of the International Conference on Multimedia and Expo ICME 2002 |
Abbreviated Journal |
|
|
|
Volume |
|
Issue |
|
Pages |
|
|
|
Keywords |
|
|
|
Abstract |
|
|
|
Address |
Lausanne |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
|
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
|
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
|
Approved |
no |
|
|
Call Number ![sorted by Call Number field, descending order (down)](img/sort_desc.gif) |
Admin @ si @ SBK2002b |
Serial |
299 |
|
Permanent link to this record |
|
|
|
|
Author |
J.M. Sanchez; X. Binefa; J.R. Kender |
![find record details (via OpenURL) openurl](img/xref.gif)
|
|
Title |
Coupled Markox Chains for Video Contents Characterization. |
Type |
Miscellaneous |
|
Year |
2002 |
Publication |
Proceeding of the International Conference on Pattern Recognition ICPR 2002 |
Abbreviated Journal |
|
|
|
Volume |
|
Issue |
|
Pages |
|
|
|
Keywords |
|
|
|
Abstract |
|
|
|
Address |
|
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
|
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
|
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
|
Approved |
no |
|
|
Call Number ![sorted by Call Number field, descending order (down)](img/sort_desc.gif) |
Admin @ si @ SBK2002a |
Serial |
298 |
|
Permanent link to this record |
|
|
|
|
Author |
Mohamed Ali Souibgui; Sanket Biswas; Sana Khamekhem Jemni; Yousri Kessentini; Alicia Fornes; Josep Llados; Umapada Pal |
![download PDF file pdf](img/file_PDF.gif)
![find record details (via OpenURL) openurl](img/xref.gif)
|
|
Title |
DocEnTr: An End-to-End Document Image Enhancement Transformer |
Type |
Conference Article |
|
Year |
2022 |
Publication |
26th International Conference on Pattern Recognition |
Abbreviated Journal |
|
|
|
Volume |
|
Issue |
|
Pages |
1699-1705 |
|
|
Keywords |
Degradation; Head; Optical character recognition; Self-supervised learning; Benchmark testing; Transformers; Magnetic heads |
|
|
Abstract |
Document images can be affected by many degradation scenarios, which cause recognition and processing difficulties. In this age of digitization, it is important to denoise them for proper usage. To address this challenge, we present a new encoder-decoder architecture based on vision transformers to enhance both machine-printed and handwritten document images, in an end-to-end fashion. The encoder operates directly on the pixel patches with their positional information without the use of any convolutional layers, while the decoder reconstructs a clean image from the encoded patches. Conducted experiments show a superiority of the proposed model compared to the state-of the-art methods on several DIBCO benchmarks. Code and models will be publicly available at: https://github.com/dali92002/DocEnTR |
|
|
Address |
August 21-25, 2022 , Montréal Québec |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
|
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
|
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
ICPR |
|
|
Notes |
DAG; 600.121; 600.162; 602.230; 600.140 |
Approved |
no |
|
|
Call Number ![sorted by Call Number field, descending order (down)](img/sort_desc.gif) |
Admin @ si @ SBJ2022 |
Serial |
3730 |
|
Permanent link to this record |
|
|
|
|
Author |
J.M. Sanchez; X. Binefa |
![find record details (via OpenURL) openurl](img/xref.gif)
|
|
Title |
Semantics from motion in news videos. |
Type |
Miscellaneous |
|
Year |
2001 |
Publication |
Proceedings of the IX Spanish Symposium on Pattern Recognition and Image Analysis, 1:79–84. |
Abbreviated Journal |
|
|
|
Volume |
|
Issue |
|
Pages |
|
|
|
Keywords |
|
|
|
Abstract |
|
|
|
Address |
|
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
|
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
|
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
|
Approved |
no |
|
|
Call Number ![sorted by Call Number field, descending order (down)](img/sort_desc.gif) |
Admin @ si @ SBi2001 |
Serial |
210 |
|
Permanent link to this record |
|
|
|
|
Author |
Mireia Sole; Joan Blanco; Debora Gil; Oliver Valero; B. Cardenas; G. Fonseka; E. Anton; Alvaro Pascual; Richard Frodsham; Zaida Sarrate |
![goto web page (via DOI) doi](img/doi.gif)
|
|
Title |
Time to match; when do homologous chromosomes become closer? |
Type |
Journal Article |
|
Year |
2022 |
Publication |
Chromosoma |
Abbreviated Journal |
CHRO |
|
|
Volume |
|
Issue |
|
Pages |
|
|
|
Keywords |
|
|
|
Abstract |
In most eukaryotes, pairing of homologous chromosomes is an essential feature of meiosis that ensures homologous recombination and segregation. However, when the pairing process begins, it is still under investigation. Contrasting data exists in Mus musculus, since both leptotene DSB-dependent and preleptotene DSB-independent mechanisms have been described. To unravel this contention, we examined homologous pairing in pre-meiotic and meiotic Mus musculus cells using a threedimensional fuorescence in situ hybridization-based protocol, which enables the analysis of the entire karyotype using DNA painting probes. Our data establishes in an unambiguously manner that 73.83% of homologous chromosomes are already paired at premeiotic stages (spermatogonia-early preleptotene spermatocytes). The percentage of paired homologous chromosomes increases to 84.60% at mid-preleptotene-zygotene stage, reaching 100% at pachytene stage. Importantly, our results demonstrate a high percentage of homologous pairing observed before the onset of meiosis; this pairing does not occur randomly, as the percentage was higher than that observed in somatic cells (19.47%) and between nonhomologous chromosomes (41.1%). Finally, we have also observed that premeiotic homologous pairing is asynchronous and independent of the chromosome size, GC content, or presence of NOR regions. |
|
|
Address |
August, 2022 |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
|
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
|
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
IAM; 601.139; 600.145; 600.096 |
Approved |
no |
|
|
Call Number ![sorted by Call Number field, descending order (down)](img/sort_desc.gif) |
Admin @ si @ SBG2022 |
Serial |
3719 |
|
Permanent link to this record |
|
|
|
|
Author |
Mireia Sole; Joan Blanco; Debora Gil; Oliver Valero; Alvaro Pascual; B. Cardenas; G. Fonseka; E. Anton; Richard Frodsham; Francesca Vidal; Zaida Sarrate |
![goto web page url](img/www.gif)
|
|
Title |
Chromosomal positioning in spermatogenic cells is influenced by chromosomal factors associated with gene activity, bouquet formation, and meiotic sex-chromosome inactivation |
Type |
Journal Article |
|
Year |
2021 |
Publication |
Chromosoma |
Abbreviated Journal |
|
|
|
Volume |
130 |
Issue |
|
Pages |
163-175 |
|
|
Keywords |
|
|
|
Abstract |
Chromosome territoriality is not random along the cell cycle and it is mainly governed by intrinsic chromosome factors and gene expression patterns. Conversely, very few studies have explored the factors that determine chromosome territoriality and its influencing factors during meiosis. In this study, we analysed chromosome positioning in murine spermatogenic cells using three-dimensionally fluorescence in situ hybridization-based methodology, which allows the analysis of the entire karyotype. The main objective of the study was to decipher chromosome positioning in a radial axis (all analysed germ-cell nuclei) and longitudinal axis (only spermatozoa) and to identify the chromosomal factors that regulate such an arrangement. Results demonstrated that the radial positioning of chromosomes during spermatogenesis was cell-type specific and influenced by chromosomal factors associated to gene activity. Chromosomes with specific features that enhance transcription (high GC content, high gene density and high numbers of predicted expressed genes) were preferentially observed in the inner part of the nucleus in virtually all cell types. Moreover, the position of the sex chromosomes was influenced by their transcriptional status, from the periphery of the nucleus when its activity was repressed (pachytene) to a more internal position when it is partially activated (spermatid). At pachytene, chromosome positioning was also influenced by chromosome size due to the bouquet formation. Longitudinal chromosome positioning in the sperm nucleus was not random either, suggesting the importance of ordered longitudinal positioning for the release and activation of the paternal genome after fertilisation. |
|
|
Address |
|
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
|
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
|
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
IAM; 600.145 |
Approved |
no |
|
|
Call Number ![sorted by Call Number field, descending order (down)](img/sort_desc.gif) |
Admin @ si @ SBG2021 |
Serial |
3592 |
|
Permanent link to this record |
|
|
|
|
Author |
Cristina Sanchez Montes; Jorge Bernal; Ana Garcia Rodriguez; Henry Cordova; Gloria Fernandez Esparrach |
![goto web page url](img/www.gif)
|
|
Title |
Revisión de métodos computacionales de detección y clasificación de pólipos en imagen de colonoscopia |
Type |
Journal Article |
|
Year |
2020 |
Publication |
Gastroenterología y Hepatología |
Abbreviated Journal |
GH |
|
|
Volume |
43 |
Issue |
4 |
Pages |
222-232 |
|
|
Keywords |
|
|
|
Abstract |
Computer-aided diagnosis (CAD) is a tool with great potential to help endoscopists in the tasks of detecting and histologically classifying colorectal polyps. In recent years, different technologies have been described and their potential utility has been increasingly evidenced, which has generated great expectations among scientific societies. However, most of these works are retrospective and use images of different quality and characteristics which are analysed off line. This review aims to familiarise gastroenterologists with computational methods and the particularities of endoscopic imaging, which have an impact on image processing analysis. Finally, the publicly available image databases, needed to compare and confirm the results obtained with different methods, are presented. |
|
|
Address |
|
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
|
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
|
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
MV; |
Approved |
no |
|
|
Call Number ![sorted by Call Number field, descending order (down)](img/sort_desc.gif) |
Admin @ si @ SBG2020 |
Serial |
3404 |
|
Permanent link to this record |
|
|
|
|
Author |
Mireia Sole; Joan Blanco; Debora Gil; G. Fonseka; Richard Frodsham; Oliver Valero; Francesca Vidal; Zaida Sarrate |
![find record details (via OpenURL) openurl](img/xref.gif)
|
|
Title |
Análisis 3d de la territorialidad cromosómica en células espermatogénicas: explorando la infertilidad desde un nuevo prisma |
Type |
Journal |
|
Year |
2017 |
Publication |
Revista Asociación para el Estudio de la Biología de la Reproducción |
Abbreviated Journal |
ASEBIR |
|
|
Volume |
22 |
Issue |
2 |
Pages |
105 |
|
|
Keywords |
|
|
|
Abstract |
|
|
|
Address |
|
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
|
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
|
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
IAM; 600.096; 600.145 |
Approved |
no |
|
|
Call Number ![sorted by Call Number field, descending order (down)](img/sort_desc.gif) |
Admin @ si @ SBG2017d |
Serial |
3042 |
|
Permanent link to this record |
|
|
|
|
Author |
Mireia Sole; Joan Blanco; Debora Gil; G. Fonseka; Richard Frodsham; Francesca Vidal; Zaida Sarrate |
![download PDF file pdf](img/file_PDF.gif)
![find record details (via OpenURL) openurl](img/xref.gif)
|
|
Title |
Noves perspectives en l estudi de la territorialitat cromosomica de cel·lules germinals masculines: estudis tridimensionals |
Type |
Journal |
|
Year |
2017 |
Publication |
Biologia de la Reproduccio |
Abbreviated Journal |
JBR |
|
|
Volume |
15 |
Issue |
|
Pages |
73-78 |
|
|
Keywords |
|
|
|
Abstract |
In somatic cells, chromosomes occupy specific nuclear regions called chromosome territories which are involved in the
maintenance and regulation of the genome. Preliminary data in male germ cells also suggest the importance of chromosome
territoriality in cell functionality. Nevertheless, the specific characteristics of testicular tissue (presence of different
cell types with different morphological characteristics, in different stages of development and with different ploidy)
makes difficult to achieve conclusive results. In this study we have developed a methodology to approach the threedimensional
study of all chromosome territories in male germ cells from C57BL/6J mice (Mus musculus). The method
includes the following steps: i) Optimized cell fixation to obtain an optimal preservation of the three-dimensionality cell
morphology, ii) Chromosome identification by FISH (Chromoprobe Multiprobe® OctoChrome™ Murine System; Cytocell)
and confocal microscopy (TCS-SP5, Leica Microsystems), iii) Cell type identification by immunofluorescence
iv) Image analysis using Matlab scripts, v) Numerical data extraction related to chromosome features, chromosome
radial position and chromosome relative position. This methodology allows the unequivocally identification and the
analysis of the chromosome territories of all spermatogenic stages. Results will provide information about the features
that determine chromosomal position, preferred associations between chromosomes, and the relationship between chromosome
positioning and genome regulation. |
|
|
Address |
|
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
|
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
|
ISBN |
978-84-697-3767-5 |
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
IAM; 600.096; 600.145 |
Approved |
no |
|
|
Call Number ![sorted by Call Number field, descending order (down)](img/sort_desc.gif) |
Admin @ si @ SBG2017c |
Serial |
2961 |
|
Permanent link to this record |
|
|
|
|
Author |
Mireia Sole; Joan Blanco; Debora Gil; G. Fonseka; Richard Frodsham; Oliver Valero; Francesca Vidal; Zaida Sarrate |
![find record details (via OpenURL) openurl](img/xref.gif)
|
|
Title |
Unraveling the enigmas of chromosome territoriality during spermatogenesis |
Type |
Conference Article |
|
Year |
2017 |
Publication |
IX Jornada del Departament de Biologia Cel•lular, Fisiologia i Immunologia |
Abbreviated Journal |
|
|
|
Volume |
|
Issue |
|
Pages |
|
|
|
Keywords |
|
|
|
Abstract |
|
|
|
Address |
UAB; Barcelona; June 2017 |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
|
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
|
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
IAM; 600.145 |
Approved |
no |
|
|
Call Number ![sorted by Call Number field, descending order (down)](img/sort_desc.gif) |
Admin @ si @ SBG2017b |
Serial |
2959 |
|
Permanent link to this record |