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Mireia Sole, Joan Blanco, Debora Gil, Oliver Valero, B. Cardenas, G. Fonseka, et al. (2022). Time to match; when do homologous chromosomes become closer? CHRO - Chromosoma, .
Abstract: In most eukaryotes, pairing of homologous chromosomes is an essential feature of meiosis that ensures homologous recombination and segregation. However, when the pairing process begins, it is still under investigation. Contrasting data exists in Mus musculus, since both leptotene DSB-dependent and preleptotene DSB-independent mechanisms have been described. To unravel this contention, we examined homologous pairing in pre-meiotic and meiotic Mus musculus cells using a threedimensional fuorescence in situ hybridization-based protocol, which enables the analysis of the entire karyotype using DNA painting probes. Our data establishes in an unambiguously manner that 73.83% of homologous chromosomes are already paired at premeiotic stages (spermatogonia-early preleptotene spermatocytes). The percentage of paired homologous chromosomes increases to 84.60% at mid-preleptotene-zygotene stage, reaching 100% at pachytene stage. Importantly, our results demonstrate a high percentage of homologous pairing observed before the onset of meiosis; this pairing does not occur randomly, as the percentage was higher than that observed in somatic cells (19.47%) and between nonhomologous chromosomes (41.1%). Finally, we have also observed that premeiotic homologous pairing is asynchronous and independent of the chromosome size, GC content, or presence of NOR regions.
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Carles Sanchez, Jorge Bernal, F. Javier Sanchez, Antoni Rosell, Marta Diez-Ferrer, & Debora Gil. (2015). Towards On-line Quantification of Tracheal Stenosis from Videobronchoscopy. IJCAR - International Journal of Computer Assisted Radiology and Surgery, 10(6), 935–945.
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Carles Sanchez, Oriol Ramos Terrades, Patricia Marquez, Enric Marti, J.Roncaries, & Debora Gil. (2015). Automatic evaluation of practices in Moodle for Self Learning in Engineering. JOTSE - Journal of Technology and Science Education, 97–106.
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Guillermo Torres, Sonia Baeza, Carles Sanchez, Ignasi Guasch, Antoni Rosell, & Debora Gil. (2022). An Intelligent Radiomic Approach for Lung Cancer Screening. APPLSCI - Applied Sciences, 12(3), 1568.
Abstract: The efficiency of lung cancer screening for reducing mortality is hindered by the high rate of false positives. Artificial intelligence applied to radiomics could help to early discard benign cases from the analysis of CT scans. The available amount of data and the fact that benign cases are a minority, constitutes a main challenge for the successful use of state of the art methods (like deep learning), which can be biased, over-fitted and lack of clinical reproducibility. We present an hybrid approach combining the potential of radiomic features to characterize nodules in CT scans and the generalization of the feed forward networks. In order to obtain maximal reproducibility with minimal training data, we propose an embedding of nodules based on the statistical significance of radiomic features for malignancy detection. This representation space of lesions is the input to a feed
forward network, which architecture and hyperparameters are optimized using own-defined metrics of the diagnostic power of the whole system. Results of the best model on an independent set of patients achieve 100% of sensitivity and 83% of specificity (AUC = 0.94) for malignancy detection.
Keywords: Lung cancer; Early diagnosis; Screening; Neural networks; Image embedding; Architecture optimization
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Guillermo Torres, Debora Gil, Antoni Rosell, S. Mena, & Carles Sanchez. (2023). Virtual Radiomics Biopsy for the Histological Diagnosis of Pulmonary Nodules – Intermediate Results of the RadioLung Project. IJCARS - International Journal of Computer Assisted Radiology and Surgery, .
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