Publicacions CVC -- Edit Record

	Publicacions CVC Home \| Show All \| Simple Search \| Advanced Search \| Add Record \| Import	You must login to submit this form! Login Quick Search: Field: contains: ...
	Edit the following record:

Author	...				is Editor
Title	...			Type
Year	...	Publication	...	Abbreviated Journal	...
Volume	...	Issue	...	Pages	...
Keywords	...
Abstract	In somatic cells, chromosomes occupy specific nuclear regions called chromosome territories which are involved in the maintenance and regulation of the genome. Preliminary data in male germ cells also suggest the importance of chromosome territoriality in cell functionality. Nevertheless, the specific characteristics of testicular tissue (presence of different cell types with different morphological characteristics, in different stages of development and with different ploidy) makes difficult to achieve conclusive results. In this study we have developed a methodology to approach the threedimensional study of all chromosome territories in male germ cells from C57BL/6J mice (Mus musculus). The method includes the following steps: i) Optimized cell fixation to obtain an optimal preservation of the three-dimensionality cell morphology, ii) Chromosome identification by FISH (Chromoprobe Multiprobe® OctoChrome™ Murine System; Cytocell) and confocal microscopy (TCS-SP5, Leica Microsystems), iii) Cell type identification by immunofluorescence iv) Image analysis using Matlab scripts, v) Numerical data extraction related to chromosome features, chromosome radial position and chromosome relative position. This methodology allows the unequivocally identification and the analysis of the chromosome territories of all spermatogenic stages. Results will provide information about the features that determine chromosomal position, preferred associations between chromosomes, and the relationship between chromosome positioning and genome regulation.
Address	...
Corporate Author	...			Thesis
Publisher	...	Place of Publication	...	Editor	...
Language	...	Summary Language	...	Original Title	...
Series Editor	...	Series Title	...	Abbreviated Series Title	...
Series Volume	...	Series Issue	...	Edition	...
ISSN	...	ISBN	...	Medium	...
Area	...	Expedition	...	Conference	...
Notes	...			Approved	yes no
Location
Call Number	...			Serial
Marked	yes no	Copy		Selected	yes no
User Keys	...
User Notes	...			User File	...
User Groups	...			Cite Key	...
Related	...
File
URL	...			DOI	...
	Online publication. Cite with this text: ...

Location Field:	my name & email address

Home

SQL Search | Library Search | Show Record | Extract Citations

Help